Genetically diverse Pseudomonas aeruginosa populations display similar transcriptomic profiles in a cystic fibrosis explanted lung

Adrian Kordes, Matthias Preusse, Sven D Willger, Peter Braubach, Danny Jonigk, Axel Haverich, Gregor Warnecke, Susanne Häussler

Abstract

Previous studies have demonstrated substantial genetic diversification of Pseudomonas aeruginosa across sub-compartments in cystic fibrosis (CF) lungs. Here, we isolate P. aeruginosa from five different sampling areas in the upper and lower airways of an explanted CF lung, analyze ex vivo transcriptional profiles by RNA-seq, and use colony re-sequencing and deep population sequencing to determine the genetic diversity within and across the various sub-compartments. We find that, despite genetic variation, the ex vivo transcriptional profiles of P. aeruginosa populations inhabiting different regions of the CF lung are similar. Although we cannot estimate the extent to which the transcriptional response recorded here actually reflects the in vivo transcriptomes, our results indicate that there may be a common in vivo transcriptional profile in the CF lung environment.

Original languageEnglish
JournalNature Communications
Volume10
Issue number1
Pages (from-to)3397
ISSN2041-1722
DOIs
Publication statusPublished - 30 Jul 2019
Externally publishedYes

Keywords

  • Bacterial Proteins/genetics
  • Cystic Fibrosis/microbiology
  • Humans
  • In Vitro Techniques
  • Lung/microbiology
  • Pseudomonas Infections/microbiology
  • Pseudomonas aeruginosa/classification
  • Transcriptome

Fingerprint

Dive into the research topics of 'Genetically diverse Pseudomonas aeruginosa populations display similar transcriptomic profiles in a cystic fibrosis explanted lung'. Together they form a unique fingerprint.

Cite this