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Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.B11

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Hansen, Susanne K ; Borland, Helena ; Hasholt, Lis F ; Tümer, Zeynep ; Nielsen, Jørgen E ; Rasmussen, Mikkel A ; Nielsen, Troels T ; Stummann, Tina C ; Fog, Karina ; Hyttel, Poul. / Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.B11. In: Stem Cell Research. 2016 ; Vol. 16, No. 3. pp. 589-92.

Bibtex

@article{2215fa187c4148a0a3e29bc76a0d7b29,
title = "Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.B11",
abstract = "Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by an expansion of the CAG-repeat in ATXN3. In this study, induced pluripotent stem cells (iPSCs) were generated from SCA3 patient dermal fibroblasts by electroporation with episomal plasmids encoding L-MYC, LIN28, SOX2, KLF4, OCT4 and short hairpin RNA targeting P53. The resulting iPSCs had normal karyotype, were free of integrated episomal plasmids, expressed pluripotency markers, could differentiate into the three germ layers in vitro and retained the disease-causing ATXN3 mutation. Potentially, this iPSC line could be a useful tool for the investigation of SCA3 disease mechanisms.",
keywords = "Journal Article",
author = "Hansen, {Susanne K} and Helena Borland and Hasholt, {Lis F} and Zeynep T{\"u}mer and Nielsen, {J{\o}rgen E} and Rasmussen, {Mikkel A} and Nielsen, {Troels T} and Stummann, {Tina C} and Karina Fog and Poul Hyttel",
note = "Copyright {\circledC} 2016 Roslin Cells Ltd. Published by Elsevier B.V. All rights reserved.",
year = "2016",
month = "5",
doi = "10.1016/j.scr.2016.02.042",
language = "English",
volume = "16",
pages = "589--92",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier BV",
number = "3",

}

RIS

TY - JOUR

T1 - Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.B11

AU - Hansen, Susanne K

AU - Borland, Helena

AU - Hasholt, Lis F

AU - Tümer, Zeynep

AU - Nielsen, Jørgen E

AU - Rasmussen, Mikkel A

AU - Nielsen, Troels T

AU - Stummann, Tina C

AU - Fog, Karina

AU - Hyttel, Poul

N1 - Copyright © 2016 Roslin Cells Ltd. Published by Elsevier B.V. All rights reserved.

PY - 2016/5

Y1 - 2016/5

N2 - Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by an expansion of the CAG-repeat in ATXN3. In this study, induced pluripotent stem cells (iPSCs) were generated from SCA3 patient dermal fibroblasts by electroporation with episomal plasmids encoding L-MYC, LIN28, SOX2, KLF4, OCT4 and short hairpin RNA targeting P53. The resulting iPSCs had normal karyotype, were free of integrated episomal plasmids, expressed pluripotency markers, could differentiate into the three germ layers in vitro and retained the disease-causing ATXN3 mutation. Potentially, this iPSC line could be a useful tool for the investigation of SCA3 disease mechanisms.

AB - Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by an expansion of the CAG-repeat in ATXN3. In this study, induced pluripotent stem cells (iPSCs) were generated from SCA3 patient dermal fibroblasts by electroporation with episomal plasmids encoding L-MYC, LIN28, SOX2, KLF4, OCT4 and short hairpin RNA targeting P53. The resulting iPSCs had normal karyotype, were free of integrated episomal plasmids, expressed pluripotency markers, could differentiate into the three germ layers in vitro and retained the disease-causing ATXN3 mutation. Potentially, this iPSC line could be a useful tool for the investigation of SCA3 disease mechanisms.

KW - Journal Article

U2 - 10.1016/j.scr.2016.02.042

DO - 10.1016/j.scr.2016.02.042

M3 - Journal article

VL - 16

SP - 589

EP - 592

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

IS - 3

ER -

ID: 48958318