Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

GABA-agonists induce the formation of low-affinity GABA-receptors on cultured cerebellar granule cells via preexisting high affinity GABA receptors

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Aspects of cAMP Signaling in Epileptogenesis and Seizures and Its Potential as Drug Target

    Research output: Contribution to journalReviewResearchpeer-review

  2. Citrate, a Ubiquitous Key Metabolite with Regulatory Function in the CNS

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. The Top 5 Neurotransmitters from a Clinical Neurologist's Perspective

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Acute hyperammonemia and systemic inflammation is associated with increased extracellular brain adenosine in rats: a biosensor study

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. A matter of balance: role of neurexin and neuroligin at the synapse

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Amitriptyline accumulation in tissues after coated activated charcoal hemoperfusion-a randomized controlled animal poisoning model

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Advanced Electrocardiogram Analysis in the Amitriptyline-poisoned Pig Treated with Activated Charcoal Haemoperfusion

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Effects of low-dose recombinant human erythropoietin treatment on cognitive performance

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Citrate, a Ubiquitous Key Metabolite with Regulatory Function in the CNS

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations
The kinetics of specific GABA-binding to membranes isolated from cerebellar granule cells, cultured for 12 days from dissociated cerebella of 7-day-old rats was studied using [3H]GABA as the ligand. The granule cells were cultured in the presence of the specific GABA receptor agonist 4, 5, 6, 7-tetrahydroisoxazolo [5,4-c]pyridin-3-ol (THIP, 150 microM) or THIP plus the antagonist bicuculline methobromide (150 microM of each) or in the absence of the agonist or antagonist. Membranes isolated from granule cells cultured in a medium without the GABA agonist revealed a single binding site for GABA with a binding constant (KD) of 7.9 +/- 0.4 nM and a Bmax of 3.42 +/- 0.08 pmol X mg-1 protein. Membranes from cells cultured in the presence of THIP had two binding sites for GABA with KD-values of 6.8 +/- 0.9 nM and 476 +/- 311 nM, respectively. The corresponding Bmax values were 4.41 +/- 0.42 pmol X mg-1 and 5.81 +/- 1.20 pmol X mg-1. The effect of culturing the cells in THIP was antagonized by the simultaneous presence of bicuculline in the culture media, i.e. no significant low-affinity binding for GABA was found on the membranes from granule cells cultured in both THIP and bicuculline. The KD value (14.3 +/- 1.4 nM) for the high affinity binding site was, however, slightly increased compared to the non-treated cells. These findings suggest that the ability of THIP to induce formation of low-affinity GABA receptors is mediated by preexisting high-affinity GABA-receptors on the granule cells.
Original languageEnglish
JournalNeurochemical Research
Volume11
Issue number4
Pages (from-to)599-606
Number of pages8
ISSN0364-3190
Publication statusPublished - 1 Apr 1986

ID: 32359253