Food as a source for quorum sensing inhibitors: iberin from horseradish revealed as a quorum sensing inhibitor of Pseudomonas aeruginosa

Tim Holm Jakobsen, Steinn Kristinn Bragason, Richard Kerry Phipps, Louise Dahl Christensen, Maria van Gennip, Morten Alhede, Mette Skindersoe, Thomas Ostenfeld Larsen, Niels Høiby, Thomas Bjarnsholt, Michael Christian Givskov

170 Citations (Scopus)

Abstract

Foods with health-promoting effects beyond nutritional values have been gaining increasing research focus in recent years, although not much has been published on this subject in relation to bacterial infections. With respect to treatment, a novel antimicrobial strategy, which is expected to transcend problems with selective pressures for antibiotic resistance, is to interrupt bacterial communication, also known as quorum sensing (QS), by means of signal antagonists, the so-called QS inhibitors (QSIs). Furthermore, QSI agents offer a potential solution to the deficiencies associated with use of traditional antibiotics to treat infections caused by bacterial biofilms and multidrug-resistant bacteria. Several QSIs of natural origin have been identified, and in this study, several common food products and plants were extracted and screened for QSI activity in an attempt to isolate and characterize previously unknown QSI compounds active against the common opportunistic pathogen Pseudomonas aeruginosa. Several extracts displayed activity, but horseradish exhibited the highest activity. Chromatographic separation led to the isolation of a potent QSI compound that was identified by liquid chromatography-diode array detector-mass spectrometry (LC-DAD-MS) and nuclear magnetic resonance (NMR) spectroscopy as iberin-an isothiocyanate produced by many members of the Brassicaceae family. Real-time PCR (RT-PCR) and DNA microarray studies showed that iberin specifically blocks expression of QS-regulated genes in P. aeruginosa.
Original languageEnglish
JournalApplied and Environmental Microbiology
Volume78
Issue number7
Pages (from-to)2410-21
Number of pages12
ISSN0099-2240
DOIs
Publication statusPublished - 2012

Keywords

  • Animals
  • Anti-Bacterial Agents
  • Armoracia
  • Bacterial Proteins
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Bacterial
  • Glycolipids
  • Humans
  • Isothiocyanates
  • Magnetic Resonance Spectroscopy
  • Mice
  • Mice, Inbred BALB C
  • Microbial Sensitivity Tests
  • Oligonucleotide Array Sequence Analysis
  • Plant Extracts
  • Pseudomonas Infections
  • Pseudomonas aeruginosa
  • Quorum Sensing
  • Real-Time Polymerase Chain Reaction

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