Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Simultaneous quantification of high-dose naloxone and naloxone-3-β-d-glucuronide in human plasma by UHPLC-MS/MS

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. The influence of sample collection methodology and sample preprocessing on the blood metabolic profile

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Development of a fast method for direct analysis of intact synthetic insulins in human plasma: the large peptide challenge

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Current strategies in the discovery of small-molecule biomarkers for Alzheimer's disease

    Research output: Contribution to journalReviewResearchpeer-review

  5. First example of hepatocyte transplantation to alleviate ornithine transcarbamylase deficiency, monitored by NMR-based metabonomics

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Differences in insulin sensitivity in the partial remission phase of childhood type 1 diabetes; a longitudinal cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Brain tumours in children and adolescents may affect the circadian rhythm and quality of life

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Brain tumours result in sleep disorders in children and adolescents

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Treatment of type 2 diabetes in children: what are the specific considerations?

    Research output: Contribution to journalReviewResearchpeer-review

View graph of relations

Aim: A novel automated serial dried blood spot (DBS) sampler, 'Fluispotter', was tested for its sampling performance. Materials & methods: An LC-MS/MS method was developed for the analysis of cortisol in DBS samples serially spotted by Fluispotter. The cortisol concentrations in 148 paired DBS and plasma samples were compared across a hematocrit (HCT) range of 22-55%. Results: The interassay accuracy and precision were <10%. Overall assay bias was negligible across the HCTs tested when analyzing the whole-spot DBS samples. The accuracy and precision of the blood volume in 10 μl DBS samples spotted by Fluispotters and micropipettes were within 3%. Deming regression and Bland-Altman analysis showed a good agreement of DBS-predicted and measured plasma cortisol. Conclusion: The Fluispotter performed serial sampling with high accuracy and precision of the sample blood volume.

Original languageEnglish
JournalBioanalysis
Volume12
Issue number10
Pages (from-to)665-681
Number of pages17
ISSN1757-6180
DOIs
Publication statusPublished - 1 May 2020

    Research areas

  • cortisol, dried blood spot, fluispotter, hematocrit, LC–MS/MS, microsampling

ID: 59978293