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Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy

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Winther, Signe Abitz ; Mannerla, Miia Maininki ; Frimodt-Møller, Marie ; Persson, Frederik ; Hansen, Tine Willum ; Lehto, Markku ; Hörkkö, Sohvi ; Blaut, Michael ; Forsblom, Carol ; Groop, Per-Henrik ; Rossing, Peter. / Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy. In: Scientific Reports. 2021 ; Vol. 11, No. 1. pp. 15208.

Bibtex

@article{5c1285ca82dd4adc855c147a15d35ec8,
title = "Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy",
abstract = "Gastrointestinal dysbiosis is common among persons with type 1 diabetes (T1D), but its potential impact on diabetic nephropathy (DN) remains obscure. We examined whether faecal biomarkers, previously associated with low-grade gastrointestinal inflammation, differ between healthy controls and T1D subjects with and without DN. Faecal samples were analyzed for levels of calprotectin, intestinal alkaline phosphatase (IAP), short-chain fatty acids (SCFA) and immunoglobulins in subjects with T1D (n = 159) and healthy controls (NDC; n = 50). The subjects with T1D were stratified based on albuminuria: normoalbuminuria (< 30 mg/g; n = 49), microalbuminuria (30-299 mg/g; n = 50) and macroalbuminuria (≥ 300 mg/g; n = 60). aecal calprotectin, IAP and immunoglobulin levels did not differ between the T1D albuminuria groups. However, when subjects were stratified based on faecal calprotectin cut-off level (50 µg/g), macroalbuminuric T1D subjects exceeded the threshold more frequently than NDC (p = 0.02). Concentrations of faecal propionate and butyrate were lower in T1D subjects compared with NDC (p = 0.04 and p = 0.03, respectively). Among T1D subjects, levels of branched SCFA (BCFA) correlated positively with current albuminuria level (isobutyrate, p = 0.03; isovalerate, p = 0.005). In our study cohort, fatty acid metabolism seemed to be altered among T1D subjects and those with albuminuria compared to NDC. This may reflect gastrointestinal imbalances associated with T1D and renal complications.",
keywords = "Aged, Biomarkers/analysis, Case-Control Studies, Cross-Sectional Studies, Diabetes Mellitus, Type 1/complications, Diabetic Nephropathies/metabolism, Fatty Acids, Volatile/analysis, Feces/chemistry, Female, Humans, Leukocyte L1 Antigen Complex/analysis, Male, Middle Aged",
author = "Winther, {Signe Abitz} and Mannerla, {Miia Maininki} and Marie Frimodt-M{\o}ller and Frederik Persson and Hansen, {Tine Willum} and Markku Lehto and Sohvi H{\"o}rkk{\"o} and Michael Blaut and Carol Forsblom and Per-Henrik Groop and Peter Rossing",
note = "{\textcopyright} 2021. The Author(s).",
year = "2021",
month = jul,
day = "26",
doi = "10.1038/s41598-021-94747-8",
language = "English",
volume = "11",
pages = "15208",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy

AU - Winther, Signe Abitz

AU - Mannerla, Miia Maininki

AU - Frimodt-Møller, Marie

AU - Persson, Frederik

AU - Hansen, Tine Willum

AU - Lehto, Markku

AU - Hörkkö, Sohvi

AU - Blaut, Michael

AU - Forsblom, Carol

AU - Groop, Per-Henrik

AU - Rossing, Peter

N1 - © 2021. The Author(s).

PY - 2021/7/26

Y1 - 2021/7/26

N2 - Gastrointestinal dysbiosis is common among persons with type 1 diabetes (T1D), but its potential impact on diabetic nephropathy (DN) remains obscure. We examined whether faecal biomarkers, previously associated with low-grade gastrointestinal inflammation, differ between healthy controls and T1D subjects with and without DN. Faecal samples were analyzed for levels of calprotectin, intestinal alkaline phosphatase (IAP), short-chain fatty acids (SCFA) and immunoglobulins in subjects with T1D (n = 159) and healthy controls (NDC; n = 50). The subjects with T1D were stratified based on albuminuria: normoalbuminuria (< 30 mg/g; n = 49), microalbuminuria (30-299 mg/g; n = 50) and macroalbuminuria (≥ 300 mg/g; n = 60). aecal calprotectin, IAP and immunoglobulin levels did not differ between the T1D albuminuria groups. However, when subjects were stratified based on faecal calprotectin cut-off level (50 µg/g), macroalbuminuric T1D subjects exceeded the threshold more frequently than NDC (p = 0.02). Concentrations of faecal propionate and butyrate were lower in T1D subjects compared with NDC (p = 0.04 and p = 0.03, respectively). Among T1D subjects, levels of branched SCFA (BCFA) correlated positively with current albuminuria level (isobutyrate, p = 0.03; isovalerate, p = 0.005). In our study cohort, fatty acid metabolism seemed to be altered among T1D subjects and those with albuminuria compared to NDC. This may reflect gastrointestinal imbalances associated with T1D and renal complications.

AB - Gastrointestinal dysbiosis is common among persons with type 1 diabetes (T1D), but its potential impact on diabetic nephropathy (DN) remains obscure. We examined whether faecal biomarkers, previously associated with low-grade gastrointestinal inflammation, differ between healthy controls and T1D subjects with and without DN. Faecal samples were analyzed for levels of calprotectin, intestinal alkaline phosphatase (IAP), short-chain fatty acids (SCFA) and immunoglobulins in subjects with T1D (n = 159) and healthy controls (NDC; n = 50). The subjects with T1D were stratified based on albuminuria: normoalbuminuria (< 30 mg/g; n = 49), microalbuminuria (30-299 mg/g; n = 50) and macroalbuminuria (≥ 300 mg/g; n = 60). aecal calprotectin, IAP and immunoglobulin levels did not differ between the T1D albuminuria groups. However, when subjects were stratified based on faecal calprotectin cut-off level (50 µg/g), macroalbuminuric T1D subjects exceeded the threshold more frequently than NDC (p = 0.02). Concentrations of faecal propionate and butyrate were lower in T1D subjects compared with NDC (p = 0.04 and p = 0.03, respectively). Among T1D subjects, levels of branched SCFA (BCFA) correlated positively with current albuminuria level (isobutyrate, p = 0.03; isovalerate, p = 0.005). In our study cohort, fatty acid metabolism seemed to be altered among T1D subjects and those with albuminuria compared to NDC. This may reflect gastrointestinal imbalances associated with T1D and renal complications.

KW - Aged

KW - Biomarkers/analysis

KW - Case-Control Studies

KW - Cross-Sectional Studies

KW - Diabetes Mellitus, Type 1/complications

KW - Diabetic Nephropathies/metabolism

KW - Fatty Acids, Volatile/analysis

KW - Feces/chemistry

KW - Female

KW - Humans

KW - Leukocyte L1 Antigen Complex/analysis

KW - Male

KW - Middle Aged

UR - http://www.scopus.com/inward/record.url?scp=85111680856&partnerID=8YFLogxK

U2 - 10.1038/s41598-021-94747-8

DO - 10.1038/s41598-021-94747-8

M3 - Journal article

C2 - 34312454

VL - 11

SP - 15208

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 15208

ER -

ID: 67683005