Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Expression of the O-Glycosylation Enzyme GalNAc-T3 in the Equatorial Segment Correlates with the Quality of Spermatozoa

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. The Effects of Dual GLP-1/GIP Receptor Agonism on Glucagon Secretion-A Review

    Research output: Contribution to journalReviewResearchpeer-review

  2. Glucagon Receptor Signaling and Glucagon Resistance

    Research output: Contribution to journalReviewResearchpeer-review

  3. Evolution and Medical Significance of LU Domain-Containing Proteins

    Research output: Contribution to journalReviewResearchpeer-review

  4. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. 5hmC Level Predicts Biochemical Failure Following Radical Prostatectomy in Prostate Cancer Patients with ERG Negative Tumors

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Characterization of Human Adrenal Steroidogenesis during Fetal Development

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Populations, decreasing fertility, and reproductive health

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. An update on semen quality among young Finnish men and comparison with Danish data

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Anogenital distance is associated with semen quality but not reproductive hormones in 1106 young men from the general population

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

We question whether the expression of GalNAc-T3, the only known O-GalNAc-transferase present in germ cells, is correlated with qualitative and functional parameters of spermatozoa. We investigated the expression of GalNAc-T3 in ejaculated spermatozoa with immunocytochemistry in swim-up purified and acrosome-reacted spermatozoa from quality-control semen donors and in semen samples from 206 randomly selected men representing a broad spectrum of semen quality. Using donor ejaculates and immunofluorescence detection we found that expression of GalNAc-T3 and the presence of the immature O-glycans Tn and T localized to the equatorial segment of spermatozoa. The proportion of GalNAc-T3-positive spermatozoa in the ejaculate increased after swim-up and appeared unaffected by induction of acrosomal exocytosis. The fraction of spermatozoa with equatorial expression of GalNAc-T3 correlated with classical semen parameters (concentration p = 9 × 10-6, morphology p = 7 × 10-8, and motility p = 1.8 × 10-5) and was significantly lower in men with oligoteratoasthenozoospermia (p = 0.0048). In conclusion, GalNAc-T3 was highly expressed by motile spermatozoa and the expression correlated positively with the classical semen parameters. Therefore, GalNAc-T3 expression seems related to the quality of the spermatozoa, and we propose that reduced expression of GalNAc-T3 may lead to impaired O-glycosylation of proteins and thereby abnormal maturation and reduced functionality of the spermatozoa.

Original languageEnglish
JournalInternational Journal of Molecular Sciences
Volume19
Issue number10
Pages (from-to)2949
ISSN1661-6596
DOIs
Publication statusPublished - 27 Sep 2018

ID: 55611577