Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Expression of the MexXY Aminoglycoside Efflux Pump and Presence of an Aminoglycoside-Modifying Enzyme in Clinical Pseudomonas aeruginosa Isolates Are Highly Correlated

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Overcoming culture restriction for SARS-CoV-2 in human cells facilitates the screening of compounds inhibiting viral replication

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Staphylococcus saprophyticus Causing Infections in Humans Is Associated with High Resistance to Heavy Metals

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Olorofim Susceptibility Testing of 1,423 Danish Mold Isolates Obtained in 2018-2019 Confirms Uniform and Broad-Spectrum Activity

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Evolution of Pseudomonas aeruginosa toward higher fitness under standard laboratory conditions

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Targeting bioenergetics is key to counteracting the drug-tolerant state of biofilm-grown bacteria

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Mining zebrafish microbiota reveals key community-level resistance against fish pathogen infection

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

The impact of MexXY efflux pump expression on aminoglycoside resistance in clinical Pseudomonas aeruginosa isolates has been debated. In this study, we found that, in general, elevated mexXY gene expression levels in clinical P. aeruginosa isolates confer to slight increases in aminoglycoside MIC levels; however, those levels rarely lead to clinically relevant resistance phenotypes. The main driver of resistance in the clinical isolates studied here was the acquisition of aminoglycoside-modifying enzymes (AMEs). Nevertheless, acquisition of an AME was strongly associated with mexY overexpression. In line with this observation, we demonstrate that the introduction of a gentamicin acetyltransferase confers to full gentamicin resistance levels in a P. aeruginosa type strain only if the MexXY efflux pump was active. We discuss that increased mexXY activity in clinical AME-harboring P. aeruginosa isolates might affect ion fluxes at the bacterial cell membrane and thus might play a role in the establishment of enhanced fitness that extends beyond aminoglycoside resistance.

Original languageEnglish
Article numbere01166-20
JournalAntimicrobial Agents and Chemotherapy
Volume65
Issue number1
ISSN0066-4804
DOIs
Publication statusPublished - Jan 2021

    Research areas

  • Aminoglycoside resistance, Aminoglycoside-modifying enzymes, Antibiotic efflux, Antibiotic resistance, MexXY

ID: 62339636