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Expression of C4.4A, a structural uPAR homolog, reflects squamous epithelial differentiation in the adult mouse and during embryogenesis

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DOI

  1. Expression of the Ly6/uPAR-domain proteins C4.4A and Haldisin in non-invasive and invasive skin lesions

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  2. The Urokinase Receptor Homolog Haldisin Is a Novel Differentiation Marker of Stratum Granulosum in Squamous Epithel

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  3. YKL-40 is differentially expressed in human embryonic stem cells and in cell progeny of the three germ layers

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  1. GPIHBP1 and Lipoprotein Lipase, Partners in Plasma Triglyceride Metabolism

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  2. Evolution and Medical Significance of LU Domain-Containing Proteins

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  3. The PCNA interaction motifs revisited: thinking outside the PIP-box

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  4. Did evolution create a flexible ligand-binding cavity in the urokinase receptor through deletion of a plesiotypic disulfide bond?

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The glycosylphosphatidylinositol (GPI)-anchored C4.4A was originally identified as a metastasis-associated protein by differential screening of rat pancreatic carcinoma cell lines. C4.4A is accordingly expressed in various human carcinoma lesions. Although C4.4A is a structural homolog of the urokinase receptor (uPAR), which is implicated in cancer invasion and metastasis, no function has so far been assigned to C4.4A. To assist future studies on its function in both physiological and pathophysiological conditions, the present study provide a global survey on C4.4A expression in the normal mouse by a comprehensive immunohistochemical mapping. This task was accomplished by staining paraffin-embedded tissues with a specific rabbit polyclonal anti-C4.4A antibody. In the adult mouse, C4.4A was predominantly expressed in the suprabasal layers of the squamous epithelia of the oral cavity, esophagus, non-glandular portion of the rodent stomach, anus, vagina, cornea, and skin. This epithelial confinement was particularly evident from the abrupt termination of C4.4A expression at the squamo-columnar transition zones found at the ano-rectal and utero-vaginal junctions, for example. During mouse embryogenesis, C4.4A expression first appears in the developing squamous epithelium at embryonic day 13.5. This anatomical location of C4.4A is thus concordant with a possible functional role in early differentiation of stratified squamous epithelia.
Original languageEnglish
JournalJournal of Histochemistry and Cytochemistry
Volume59
Issue number2
Pages (from-to)188-201
Number of pages14
ISSN0022-1554
DOIs
Publication statusPublished - 2011

    Research areas

  • Animals, Cell Adhesion Molecules, Cell Differentiation, Embryonic Development, Epithelial Cells, Female, GPI-Linked Proteins, Gene Expression Regulation, Developmental, Humans, Immunohistochemistry, Male, Mice, Phenotype, Protein Transport, Rats, Receptors, Urokinase Plasminogen Activator, Reproduction, Sequence Homology, Amino Acid

ID: 33285872