Abstract
BACKGROUND: To evaluate the hypothesis that gastrin is a local growth factor in colonic carcinomas, the expression of gastrin messenger RNA (mRNA) and peptides were examined in five human colon carcinoma cell lines, 12 solid colon carcinomas, and normal colonic tissue.
METHODS: Northern analysis, reverse-transcription PCR, and a library of sequence-specific radioimmunoassays were the principal methods.
RESULTS: Cell lines, tumors, and normal tissue all expressed a gastrin mRNA of 0.7 kilobases, and all cell lines contained incompletely processed progastrin (range, 17-54 fmol/10(6) cells). Two cell lines secreted progastrin into the media (LoVo, 25 +/- 3 pmol/L; HCT116; 12 +/- 2 pmol/L). Normal colonic tissue and all the solid tumors also contained progastrin, the concentration being higher in tumors (range, 0.4-2 pmol/g) than in normal tissue (range, 0.1-0.2 pmol/g). Only one tumor contained carboxyamidated gastrins.
CONCLUSIONS: Normal and neoplastic colonic mucosa both express the gastrin gene, but the posttranslational phase of expression is attenuated. The incomplete processing and low level of expression suggest that autocrine gastrin secretion has only minor significance for normal adult and most neoplastic colonic tissue.
Original language | English |
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Journal | Gastroenterology |
Volume | 104 |
Issue number | 4 |
Pages (from-to) | 1099-107 |
Number of pages | 9 |
ISSN | 0016-5085 |
DOIs | |
Publication status | Published - Apr 1993 |
Externally published | Yes |
Keywords
- Actins/genetics
- Adenocarcinoma/metabolism
- Blotting, Northern
- Colon/metabolism
- Colonic Neoplasms/metabolism
- Colorectal Neoplasms/metabolism
- Exons
- Gastric Mucosa/metabolism
- Gastrins/genetics
- Humans
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction/methods
- Protein Precursors/genetics
- RNA Probes
- RNA, Messenger/analysis
- Rectal Neoplasms/metabolism
- Tumor Cells, Cultured