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Exploring the evolution and epidemiology of European CC1-MRSA-IV: tracking a multidrug-resistant community-associated meticillin-resistant Staphylococcus aureus clone

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  • Megan R Earls
  • Eike J Steinig
  • Stefan Monecke
  • José A Samaniego Castruita
  • Alexandra Simbeck
  • Wulf Schneider-Brachert
  • Teodora Vremerǎ
  • Olivia S Dorneanu
  • Igor Loncaric
  • Michèle Bes
  • Alicia Lacoma
  • Cristina Prat Aymerich
  • Ulrich Wernery
  • Marc Armengol-Porta
  • Anita Blomfeldt
  • Sebastian Duchene
  • Mette D Bartels
  • Ralf Ehricht
  • David C Coleman
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This study investigated the evolution and epidemiology of the community-associated and multidrug-resistant Staphylococcus aureus clone European CC1-MRSA-IV. Whole-genome sequences were obtained for 194 European CC1-MRSA-IV isolates (189 of human and 5 of animal origin) from 12 countries, and 10 meticillin-susceptible precursors (from North-Eastern Romania; all of human origin) of the clone. Phylogenetic analysis was performed using a maximum-likelihood approach, a time-measured phylogeny was reconstructed using Bayesian analysis, and in silico microarray genotyping was performed to identify resistance, virulence-associated and SCCmec (staphylococcal cassette chromosome mec) genes. Isolates were typically sequence type 1 (190/204) and spa type t127 (183/204). Bayesian analysis indicated that European CC1-MRSA-IV emerged in approximately 1995 before undergoing rapid expansion in the late 1990s and 2000s, while spreading throughout Europe and into the Middle East. Phylogenetic analysis revealed an unstructured meticillin-resistant S. aureus (MRSA) population, lacking significant geographical or temporal clusters. The MRSA were genotypically multidrug-resistant, consistently encoded seh, and intermittently (34/194) encoded an undisrupted hlb gene with concomitant absence of the lysogenic phage-encoded genes sak and scn. All MRSA also harboured a characteristic ~5350 nt insertion in SCCmec adjacent to orfX. Detailed demographic data from Denmark showed that there, the clone is typically (25/35) found in the community, and often (10/35) among individuals with links to South-Eastern Europe. This study elucidated the evolution and epidemiology of European CC1-MRSA-IV, which emerged from a meticillin-susceptible lineage prevalent in North-Eastern Romania before disseminating rapidly throughout Europe.

Original languageEnglish
Article number000601
JournalMicrobial genomics
Volume7
Issue number7
Pages (from-to)1-9
Number of pages9
ISSN2057-5858
DOIs
Publication statusPublished - Jul 2021

    Research areas

  • CA-MRSA, European CC1-MRSA-IV clone, epidemiology, evolution, phylogenomics, transmission

ID: 66572190