Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Ex vivo diffusion-weighted MRI tractography of the Göttingen minipig limbic system

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Brain resting-state connectivity in the development of secondary hyperalgesia in healthy men

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Topological principles and developmental algorithms might refine diffusion tractography

    Research output: Contribution to journalReviewpeer-review

  3. The number of neurons in specific amygdala regions is associated with boldness in mink: a study in animal personality

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. No detectable effect on visual responses using functional MRI in a rodent model of α-synuclein expression

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. On the cortical connectivity in the macaque brain: a comparison of diffusion tractography and histological tracing data

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Functional and Structural Plasticity Co-express in a Left Premotor Region During Early Bimanual Skill Learning

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Streamline tractography for 3D mapping of axon bundle organization in one MRI voxel using ultra-high resolution synchrotron radiation imaging

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

  • Johannes Bech
  • Dariusz Orlowski
  • Andreas N Glud
  • Tim B Dyrby
  • Jens Christian H Sørensen
  • Carsten R Bjarkam
View graph of relations

The limbic system encompasses a collection of brain areas primarily involved in higher cognitive and emotional processing. Altered function in the limbic circuitry may play a major role in various psychiatric disorders. This study aims to provide a high-quality ex vivo diffusion-weighted MRI (DWI) tractographic overview of the Göttingen minipig limbic system pathways, which are currently not well described. This may facilitate future translational large animal studies. The study used previously obtained post-mortem DWI scans in 3 female Göttingen minipigs aging 11-15 months. The tractography performed on the DWI data set was made using a probabilistic algorithm, and regions of interest (ROIs) were defined in accordance with a histological atlas. The investigated pathways included the fornix, mammillothalamic tract, stria terminalis, stria medullaris, habenulo-interpeduncular tract, and cingulum. All the investigated limbic connections could be visualized with a high detail yielding a comprehensive three-dimensional overview, which was emphasized by the inclusion of video material. The minipig limbic system pathways displayed using tractography closely resembled what was previously described in both human studies and neuronal tracing studies from other mammalian species. We encountered well-known inherent methodological challenges of tractography, e.g., partial volume effects and complex white matter regions, which may have contributed to derouted false-positive streamlines and the failure to visualize some of the minor limbic pathway ramifications. This underlines the importance of preexisting anatomical knowledge. Conclusively, we have, for the first time, provided an overview and substantial insight of the Göttingen minipig limbic system.

Original languageEnglish
JournalBrain structure & function
Volume225
Issue number3
Pages (from-to)1055-1071
Number of pages17
ISSN0177-5154
DOIs
Publication statusPublished - Apr 2020

    Research areas

  • Cingulum, Fornix, Mammillothalamic tract, Stria medullaris, Stria terminalis, Sus scrofa

ID: 59657015