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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Evolution of Pseudomonas aeruginosa toward higher fitness under standard laboratory conditions

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  2. Ecological succession in the vaginal microbiota during pregnancy and birth

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  4. Low spatial structure and selection against secreted virulence factors attenuates pathogenicity in Pseudomonas aeruginosa

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  1. Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth

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  2. Targeting bioenergetics is key to counteracting the drug-tolerant state of biofilm-grown bacteria

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  3. Mining zebrafish microbiota reveals key community-level resistance against fish pathogen infection

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Identifying genetic factors that contribute to the evolution of adaptive phenotypes in pathogenic bacteria is key to understanding the establishment of infectious diseases. In this study, we performed mutation accumulation experiments to record the frequency of mutations and their effect on fitness in hypermutator strains of the environmental bacterium Pseudomonas aeruginosa in comparison to the host-niche-adapted Salmonella enterica. We demonstrate that P. aeruginosa, but not S. enterica, hypermutators evolve toward higher fitness under planktonic conditions. Adaptation to increased growth performance was accompanied by a reversible perturbing of the local genetic context of membrane and cell wall biosynthesis genes. Furthermore, we observed a fine-tuning of complex regulatory circuits involving multiple di-guanylate modulating enzymes that regulate the transition between fast growing planktonic and sessile biofilm-associated lifestyles. The redundancy and local specificity of the di-guanylate signaling pathways seem to allow a convergent shift toward increased growth performance across niche-adapted clonal P. aeruginosa lineages, which is accompanied by a pronounced heterogeneity of their motility, virulence, and biofilm phenotypes.

Original languageEnglish
JournalISME Journal
Volume15
Issue number4
Pages (from-to)1165-1177
Number of pages13
ISSN1751-7362
DOIs
Publication statusPublished - Apr 2021

ID: 62339804