Evidence favoring the involvement of CC chemokine receptor (CCR) 5 in T-lymphocyte accumulation in optic neuritis

T L Sørensen; R M Ransohoff; J Jensen; F Sellebjerg

doi:10.1034/j.1600-0404.2003.01372.x

Evidence favoring the involvement of CC chemokine receptor (CCR) 5 in T-lymphocyte accumulation in optic neuritis

T L Sørensen, R M Ransohoff, J Jensen, F Sellebjerg

5 Citations (Scopus)

Abstract

OBJECTIVE: To define the relationships between levels of chemokine receptor (CCR)5+ T-cells in blood and cerebrospinal fluid (CSF) of optic neuritis (ON) and control patients (CON).

MATERIALS AND METHODS: Expression of CCR5 and related receptors CCR1 and CCR3 on CD4- and CD8-positive T-cells in peripheral blood mononuclear cells (PBMC) and CSF was determined by flow cytometry in 20 patients with ON, 16 control patients with lumbar spondylosis, 20 healthy controls (HC) and 16 patients with rheumatoid arthritis (RA).

RESULTS: CCR5+CD4+ and CD8+ T-cells were enriched in CSF, compared with PBMC, in both ON and CON patients (all P < 0.001), and the percentages of CD4+/CCR5+ (r = 0.917) and CD8+/CCR5+ (r = 0.828) cells in PBMC and CSF were strongly and directly correlated. CCR5+ T-cells produce high amounts of tumor necrosis factor-alpha (TNF-alpha) and very low amounts of interleukin-5 (IL-5). Closely related receptors (CCR1, CCR3) were not altered.

CONCLUSIONS: Our data suggest an involvement of CCR5 in T-cell accumulation in the inflamed central nervous system.

Original language	English
Journal	Acta Neurologica Scandinavica
Volume	107
Issue number	3
Pages (from-to)	221-7
Number of pages	7
ISSN	0001-6314
DOIs	https://doi.org/10.1034/j.1600-0404.2003.01372.x
Publication status	Published - Mar 2003
Externally published	Yes

Keywords

Adult
Aged
Female
Flow Cytometry
Humans
Male
Middle Aged
Optic Neuritis/immunology
Receptors, CCR5/biosynthesis
T-Lymphocytes/immunology
Tumor Necrosis Factor-alpha/biosynthesis

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10.1034/j.1600-0404.2003.01372.x

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@article{16746c96a23344e385b557e10ae7cc5e,

title = "Evidence favoring the involvement of CC chemokine receptor (CCR) 5 in T-lymphocyte accumulation in optic neuritis",

abstract = "OBJECTIVE: To define the relationships between levels of chemokine receptor (CCR)5+ T-cells in blood and cerebrospinal fluid (CSF) of optic neuritis (ON) and control patients (CON).MATERIALS AND METHODS: Expression of CCR5 and related receptors CCR1 and CCR3 on CD4- and CD8-positive T-cells in peripheral blood mononuclear cells (PBMC) and CSF was determined by flow cytometry in 20 patients with ON, 16 control patients with lumbar spondylosis, 20 healthy controls (HC) and 16 patients with rheumatoid arthritis (RA).RESULTS: CCR5+CD4+ and CD8+ T-cells were enriched in CSF, compared with PBMC, in both ON and CON patients (all P < 0.001), and the percentages of CD4+/CCR5+ (r = 0.917) and CD8+/CCR5+ (r = 0.828) cells in PBMC and CSF were strongly and directly correlated. CCR5+ T-cells produce high amounts of tumor necrosis factor-alpha (TNF-alpha) and very low amounts of interleukin-5 (IL-5). Closely related receptors (CCR1, CCR3) were not altered.CONCLUSIONS: Our data suggest an involvement of CCR5 in T-cell accumulation in the inflamed central nervous system.",

keywords = "Adult, Aged, Female, Flow Cytometry, Humans, Male, Middle Aged, Optic Neuritis/immunology, Receptors, CCR5/biosynthesis, T-Lymphocytes/immunology, Tumor Necrosis Factor-alpha/biosynthesis",

author = "S{\o}rensen, \{T L\} and Ransohoff, \{R M\} and J Jensen and F Sellebjerg",

year = "2003",

month = mar,

doi = "10.1034/j.1600-0404.2003.01372.x",

language = "English",

volume = "107",

pages = "221--7",

journal = "Acta Neurologica Scandinavica",

issn = "0001-6314",

publisher = "John Wiley and Sons Inc",

number = "3",

}

TY - JOUR

T1 - Evidence favoring the involvement of CC chemokine receptor (CCR) 5 in T-lymphocyte accumulation in optic neuritis

AU - Sørensen, T L

AU - Ransohoff, R M

AU - Jensen, J

AU - Sellebjerg, F

PY - 2003/3

Y1 - 2003/3

N2 - OBJECTIVE: To define the relationships between levels of chemokine receptor (CCR)5+ T-cells in blood and cerebrospinal fluid (CSF) of optic neuritis (ON) and control patients (CON).MATERIALS AND METHODS: Expression of CCR5 and related receptors CCR1 and CCR3 on CD4- and CD8-positive T-cells in peripheral blood mononuclear cells (PBMC) and CSF was determined by flow cytometry in 20 patients with ON, 16 control patients with lumbar spondylosis, 20 healthy controls (HC) and 16 patients with rheumatoid arthritis (RA).RESULTS: CCR5+CD4+ and CD8+ T-cells were enriched in CSF, compared with PBMC, in both ON and CON patients (all P < 0.001), and the percentages of CD4+/CCR5+ (r = 0.917) and CD8+/CCR5+ (r = 0.828) cells in PBMC and CSF were strongly and directly correlated. CCR5+ T-cells produce high amounts of tumor necrosis factor-alpha (TNF-alpha) and very low amounts of interleukin-5 (IL-5). Closely related receptors (CCR1, CCR3) were not altered.CONCLUSIONS: Our data suggest an involvement of CCR5 in T-cell accumulation in the inflamed central nervous system.

AB - OBJECTIVE: To define the relationships between levels of chemokine receptor (CCR)5+ T-cells in blood and cerebrospinal fluid (CSF) of optic neuritis (ON) and control patients (CON).MATERIALS AND METHODS: Expression of CCR5 and related receptors CCR1 and CCR3 on CD4- and CD8-positive T-cells in peripheral blood mononuclear cells (PBMC) and CSF was determined by flow cytometry in 20 patients with ON, 16 control patients with lumbar spondylosis, 20 healthy controls (HC) and 16 patients with rheumatoid arthritis (RA).RESULTS: CCR5+CD4+ and CD8+ T-cells were enriched in CSF, compared with PBMC, in both ON and CON patients (all P < 0.001), and the percentages of CD4+/CCR5+ (r = 0.917) and CD8+/CCR5+ (r = 0.828) cells in PBMC and CSF were strongly and directly correlated. CCR5+ T-cells produce high amounts of tumor necrosis factor-alpha (TNF-alpha) and very low amounts of interleukin-5 (IL-5). Closely related receptors (CCR1, CCR3) were not altered.CONCLUSIONS: Our data suggest an involvement of CCR5 in T-cell accumulation in the inflamed central nervous system.

KW - Adult

KW - Aged

KW - Female

KW - Flow Cytometry

KW - Humans

KW - Male

KW - Middle Aged

KW - Optic Neuritis/immunology

KW - Receptors, CCR5/biosynthesis

KW - T-Lymphocytes/immunology

KW - Tumor Necrosis Factor-alpha/biosynthesis

UR - https://www.scopus.com/pages/publications/0344407027

U2 - 10.1034/j.1600-0404.2003.01372.x

DO - 10.1034/j.1600-0404.2003.01372.x

M3 - Journal article

C2 - 12614317

SN - 0001-6314

VL - 107

SP - 221

EP - 227

JO - Acta Neurologica Scandinavica

JF - Acta Neurologica Scandinavica

IS - 3

ER -

Evidence favoring the involvement of CC chemokine receptor (CCR) 5 in T-lymphocyte accumulation in optic neuritis

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