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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Evaluation of Circulating miRNA Biomarkers of Testicular Germ Cell Tumors during Therapy and Follow-up-A Copenhagen Experience

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DOI

  1. Luteinizing Hormone Receptor Is Expressed in Testicular Germ Cell Tumors: Possible Implications for Tumor Growth and Prognosis

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Real-World Impact of Immune Checkpoint Inhibitors in Metastatic Uveal Melanoma

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  3. Predicting visual acuity deterioration and radiation-induced toxicities after brachytherapy for choroidal melanomas

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  1. Growth and Adult Height in Girls With Turner Syndrome Following IGF-1 Titrated Growth Hormone Treatment

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  2. Sexual Function and Quality of Life in a National Cohort of Survivors of Bilateral Testicular Cancer

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  3. Activin A determines steroid levels and composition in the fetal testis

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Distinguishing between hidden testes and anorchia: The role of endocrine evaluation in infancy and childhood

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  5. Heritability of pubertal timing: detailed evaluation of specific milestones in healthy boys and girls

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New microRNA-based serum biomarkers (miRNA-367-3p, -371a-3p, -372-3p, and -373-3p) have shown great potential for the detection of testicular germ cell tumors (TGCTs), but few studies have investigated the clinical utility and performance of these tests in treatment monitoring. In this study, circulating miRNA levels were measured, together with serum tumor markers alpha-fetoprotein (AFP), β-subunit of human chorionic gonadotropin (β-HCG) and lactate dehydrogenase (LDH) in 406 consecutive blood samples obtained during the treatment and follow-up of 52 TGCT patients at the Copenhagen University Hospital. After testing three different methods of RNA isolation from peripheral blood and PCR quantification in a subset of samples (n = 15), the best performing setup of targeted isolation of miRNAs inside and outside exosomes was selected to analyze all samples. At primary diagnosis, the miRNAs significantly outperformed the serum tumor markers, with a sensitivity and specificity of 78% and 100% (based on 40 patients), respectively. The picture was not as clear when patient trajectories were investigated, with both positive and negative signals for miRNAs and serum tumor markers. To establish whether measuring miRNAs adds value beyond the primary diagnosis, large prospective clinical trials comparing miRNAs and classical tumor markers during the treatment and follow-up of TGCT patients are needed.

Original languageEnglish
JournalCancers
Volume12
Issue number3
ISSN2072-6694
DOIs
Publication statusPublished - 23 Mar 2020

ID: 59966114