Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Evaluation of a new commercial assay for diagnosis of pulmonary and nonpulmonary tuberculosis

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Community-acquired meningitis caused by beta-haemolytic streptococci in adults: a nationwide population-based cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Multicenter evaluation of the new QIAstat Gastrointestinal Panel for the rapid syndromic testing of acute gastroenteritis

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. In-hospital metabolite changes in infective endocarditis-a longitudinal 1H NMR-based study

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Eradicating MRSA carriage: the impact of throat carriage and Panton-Valentine leukocidin genes on success rates

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. The effect of short-course gentamicin therapy on kidney function in patients with bacteraemia-a retrospective cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Characteristics of patients with familial Mediterranean fever in Denmark: a retrospective nationwide register-based cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Remdesivir til behandling af COVID-19-pneumoni

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Children exposed or unexposed to HIV: weight, height and BMI during the first five years of life. A Danish Nationwide Cohort Study

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Risk factors during pregnancy and birth-related complications in HIV-positive versus HIV-negative women in Denmark, 2002-2014

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations
A new commercial assay for the diagnosis of tuberculosis, the BDProbeTec ET Direct Detection assay (Becton Dickinson, USA), was evaluated using 351 respiratory and 372 nonrespiratory specimens. The results were compared to detection of Mycobacterium tuberculosis complex (MTC) by conventional culture. Among the 351 respiratory specimens, MTC bacteria were identified in 150, of which 85 were positive by both microscopy and the assay. Sixty-five specimens culture positive for MTC were microscopy negative; of these, 39 were positive in the assay. All 26 specimens culture positive for nontuberculous mycobacteria (NTM) were negative by the assay. Of 175 specimens culture negative for MTC, 3 were falsely positive by the assay and 1 yielded inhibition. The overall sensitivity and specificity values were 82.7% and 98.5%, respectively. The sensitivity for microscopy-positive and -negative respiratory specimens was 100% and 60%, respectively. After correction for discrepancies, the specificity was 99% compared with notification data. The BDProbeTec ET assay detected 66 of 67 microscopy-positive and 50 of 125 microscopy-negative nonrespiratory specimens. The result for one specimen was inconclusive. All nine specimens containing NTM were negative by the assay. Of 171 specimens culture negative for MTC, 6 were falsely positive by the assay. The overall sensitivity and specificity values obtained with nonrespiratory specimens were 60.7% and 96.7%, respectively. After examining discrepancies by reviewing the patients' histories, the specificity was 98.9%. The sensitivity was 98.5% in microscopy-positive specimens and 40.3% in microscopy-negative specimens. The overall inhibition rate was 0.3%. The BDProbeTec ET assay is a fast, effective, and user-friendly system that can be used for rapid detection of MTC bacteria in respiratory and microscopy-positive nonrespiratory specimens as an important supplement to smear and culture.
Original languageEnglish
JournalEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
Volume21
Issue number6
Pages (from-to)455-60
Number of pages6
ISSN0934-9723
DOIs
Publication statusPublished - Jun 2002

    Research areas

  • Bacteriological Techniques, Body Fluids, Culture Media, Feces, Humans, Mycobacterium tuberculosis, Reagent Kits, Diagnostic, Respiratory System, Sensitivity and Specificity, Suppuration, Tuberculosis, Tuberculosis, Pulmonary, Wounds and Injuries

ID: 39873790