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Erythrocytes restrict microvesicle-induced production of reactive oxygen species by polymorphonuclear leukocytes

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Microvesicles (MVs) are extracellular vesicles released by several cell types upon activation or apoptosis. MVs have the potential to activate complement, which has been suggested to mediate their clearance. However, it is not clear how complement-opsonized MVs are prevented from activating circulating polymorphonuclear leukocytes (PMNs) with release of reactive oxygen species (ROS) and potential damage of endothelium and other bystander cells as consequence. We hypothesized that binding of opsonized MVs to erythrocytes (Es) attenuates MV-induced PMN activation. To test this, normal PMNs were exposed to MVs in the presence and absence of Es from allogenic healthy donors. As analyzed by flow cytometry, the presence of Es restricted the PMN binding of MVs by about 85% (p = 0.002) and mediated a 60-70% inhibition of the PMN production of the ROS H2 O2 , induced by MVs, when lipopolysaccharide was used as a primer (p = 0.002). The competitive binding of MVs to Es was partly dependent on complement, since EDTA inhibited MV binding to Es by 75%. These data suggest that Es, through competitive binding, may restrict MV-induced activation of circulating PMNs and thereby serve a role as a regulator of PMN activation.

Original languageEnglish
JournalAPMIS - Journal of Pathology, Microbiology and Immunology
Volume127
Issue number7
Pages (from-to)538-542
Number of pages5
ISSN0903-4641
DOIs
Publication statusPublished - Jul 2019

    Research areas

  • Adult, Cell-Derived Microparticles/metabolism, Endothelium/metabolism, Erythrocytes/metabolism, Female, Flow Cytometry/methods, Humans, Leukocyte Count/methods, Male, Reactive Oxygen Species/metabolism, Young Adult

ID: 58901428