Abstract
Microvesicles (MVs) are extracellular vesicles released by several cell types upon activation or apoptosis. MVs have the potential to activate complement, which has been suggested to mediate their clearance. However, it is not clear how complement-opsonized MVs are prevented from activating circulating polymorphonuclear leukocytes (PMNs) with release of reactive oxygen species (ROS) and potential damage of endothelium and other bystander cells as consequence. We hypothesized that binding of opsonized MVs to erythrocytes (Es) attenuates MV-induced PMN activation. To test this, normal PMNs were exposed to MVs in the presence and absence of Es from allogenic healthy donors. As analyzed by flow cytometry, the presence of Es restricted the PMN binding of MVs by about 85% (p = 0.002) and mediated a 60-70% inhibition of the PMN production of the ROS H2 O2 , induced by MVs, when lipopolysaccharide was used as a primer (p = 0.002). The competitive binding of MVs to Es was partly dependent on complement, since EDTA inhibited MV binding to Es by 75%. These data suggest that Es, through competitive binding, may restrict MV-induced activation of circulating PMNs and thereby serve a role as a regulator of PMN activation.
Original language | English |
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Journal | APMIS - Journal of Pathology, Microbiology and Immunology |
Volume | 127 |
Issue number | 7 |
Pages (from-to) | 538-542 |
Number of pages | 5 |
ISSN | 0903-4641 |
DOIs | |
Publication status | Published - Jul 2019 |
Keywords
- Adult
- Cell-Derived Microparticles/metabolism
- Endothelium/metabolism
- Erythrocytes/metabolism
- Female
- Flow Cytometry/methods
- Humans
- Leukocyte Count/methods
- Male
- Reactive Oxygen Species/metabolism
- Young Adult