Enzyme-Linked Immunosorbent Assay for Activation of the Classical Complement Pathway by Plasmodium falciparum-Infected Erythrocyte Surface Antigen-Specific Antibodies

Mads Delbo Larsen; Rafael Bayarri-Olmos; Peter Garred; Lars Hviid

doi:10.1007/978-1-0716-2189-9_50

Enzyme-Linked Immunosorbent Assay for Activation of the Classical Complement Pathway by Plasmodium falciparum-Infected Erythrocyte Surface Antigen-Specific Antibodies

Mads Delbo Larsen
, Rafael Bayarri-Olmos
, Peter Garred
, Lars Hviid^*

^*Corresponding author for this work

1 Citation (Scopus)

Abstract

Enzyme-linked immunosorbent assays (ELISA) have a wide range of applications, ranging from specific antibody titer determination to quantification of any biological or non-biological substance with a specific binding partner (usually an antibody). The activity of biological cascades, such as the complement cascade of the innate immune system, can also be assessed by ELISA. We present here an assay optimized for the detection of the activation of the classical complement pathway by polyclonal and monoclonal antibodies (mAbs) specific for Plasmodium falciparum-infected erythrocyte surface antigens.

Original language	English
Journal	Methods in Molecular Biology
Volume	2470
Pages (from-to)	673-678
Number of pages	6
ISSN	1064-3745
DOIs	https://doi.org/10.1007/978-1-0716-2189-9_50
Publication status	Published - 2022

Keywords

Antibodies
Complement
ELISA
Malaria
PfEMP1

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10.1007/978-1-0716-2189-9_50

Cite this

Enzyme-Linked Immunosorbent Assay for Activation of the Classical Complement Pathway by Plasmodium falciparum-Infected Erythrocyte Surface Antigen-Specific Antibodies. / Larsen, Mads Delbo; Bayarri-Olmos, Rafael; Garred, Peter et al.
In: Methods in Molecular Biology, Vol. 2470, 2022, p. 673-678.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "Enzyme-linked immunosorbent assays (ELISA) have a wide range of applications, ranging from specific antibody titer determination to quantification of any biological or non-biological substance with a specific binding partner (usually an antibody). The activity of biological cascades, such as the complement cascade of the innate immune system, can also be assessed by ELISA. We present here an assay optimized for the detection of the activation of the classical complement pathway by polyclonal and monoclonal antibodies (mAbs) specific for Plasmodium falciparum-infected erythrocyte surface antigens.",

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author = "Larsen, \{Mads Delbo\} and Rafael Bayarri-Olmos and Peter Garred and Lars Hviid",

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doi = "10.1007/978-1-0716-2189-9\_50",

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AU - Larsen, Mads Delbo

AU - Bayarri-Olmos, Rafael

AU - Garred, Peter

AU - Hviid, Lars

PY - 2022

Y1 - 2022

N2 - Enzyme-linked immunosorbent assays (ELISA) have a wide range of applications, ranging from specific antibody titer determination to quantification of any biological or non-biological substance with a specific binding partner (usually an antibody). The activity of biological cascades, such as the complement cascade of the innate immune system, can also be assessed by ELISA. We present here an assay optimized for the detection of the activation of the classical complement pathway by polyclonal and monoclonal antibodies (mAbs) specific for Plasmodium falciparum-infected erythrocyte surface antigens.

AB - Enzyme-linked immunosorbent assays (ELISA) have a wide range of applications, ranging from specific antibody titer determination to quantification of any biological or non-biological substance with a specific binding partner (usually an antibody). The activity of biological cascades, such as the complement cascade of the innate immune system, can also be assessed by ELISA. We present here an assay optimized for the detection of the activation of the classical complement pathway by polyclonal and monoclonal antibodies (mAbs) specific for Plasmodium falciparum-infected erythrocyte surface antigens.

KW - Antibodies

KW - Complement

KW - ELISA

KW - Malaria

KW - PfEMP1

UR - https://www.scopus.com/pages/publications/85135377227

U2 - 10.1007/978-1-0716-2189-9_50

DO - 10.1007/978-1-0716-2189-9_50

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SN - 1064-3745

VL - 2470

SP - 673

EP - 678

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Enzyme-Linked Immunosorbent Assay for Activation of the Classical Complement Pathway by Plasmodium falciparum-Infected Erythrocyte Surface Antigen-Specific Antibodies

Abstract

Keywords

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