Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Enteroaggregative Escherichia coli O78:H10, the cause of an outbreak of urinary tract infection

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Update from a twelve-year nationwide fungaemia surveillance: increasing intrinsic and acquired resistance causes concern

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. PREVALENCE OF HUMAN PAPILLOMAVIRUS IN SELF-TAKEN SAMPLES FROM SCREENING NON-ATTENDERS

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Dientamoeba fragilis - a Commensal in Children in Danish Day Care Centers

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Differential detection of Human Papillomavirus genotypes and cervical intraepithelial neoplasia by four commercial assays

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Treatment of candidemia in a nationwide setting: increased survival with primary echinocandin treatment

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Sequence Type 410 Is Causing New International High-Risk Clones

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Update from a twelve-year nationwide fungaemia surveillance: increasing intrinsic and acquired resistance causes concern

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Incidence of HACEK bacteraemia in Denmark: A 6-year population-based study

    Research output: Contribution to journalJournal articleResearchpeer-review

  • Bente Olesen
  • Flemming Scheutz
  • Rebecca L Andersen
  • Megan Menard
  • Nadia Boisen
  • Brian Johnston
  • Dennis S Hansen
  • Karen Krogfelt
  • James P Nataro
  • James R Johnson
View graph of relations
In 1991, multiresistant Escherichia coli O78:H10 strains caused an outbreak of urinary tract infections in Copenhagen, Denmark. The phylogenetic origin, clonal background, and virulence characteristics of the outbreak isolates, and their relationship to nonoutbreak O78:H10 strains according to these traits and resistance profiles, are unknown. Accordingly, we extensively characterized 51 archived E. coli O78:H10 isolates (48 human isolates from seven countries, including 19 Copenhagen outbreak isolates, and 1 each of calf, avian, and unknown-source isolates), collected from 1956 through 2000. E. coli O78:H10 was clonally heterogeneous, comprising one dominant clonal group (61% of isolates, including all 19 outbreak isolates) from ST10 (phylogenetic group A) plus several minor clonal groups (phylogenetic groups A and D). All ST10 isolates, versus 25% of non-ST10 isolates, were identified by molecular methods as enteroaggregative E. coli (EAEC) (P <0.001). Genes present in >90% of outbreak isolates included fimH (type 1 fimbriae; ubiquitous in E. coli); fyuA, traT, and iutA (associated with extraintestinal pathogenic E. coli [ExPEC]); and sat, pic, aatA, aggR, aggA, ORF61, aaiC, aap, and ORF3 (associated with EAEC). An outbreak isolate was lethal in a murine subcutaneous sepsis model and exhibited characteristic EAEC "stacked brick" adherence to cultured epithelial cells. Thus, the 1991 Copenhagen outbreak was caused by a tight, non-animal-associated subset within a broadly disseminated O78:H10 clonal group (ST10; phylogenetic group A), members of which exhibit both ExPEC and EAEC characteristics, whereas O78:H10 isolates overall are phylogenetically diverse. Whether ST10 O78:H10 EAEC strains are both uropathogenic and diarrheagenic warrants further investigation.
Original languageEnglish
JournalJournal of Clinical Microbiology
Volume50
Issue number11
Pages (from-to)3703-11
Number of pages9
ISSN0095-1137
DOIs
Publication statusPublished - 2012

ID: 36911430