Endothelial nitric oxide synthase phosphorylation at Threonine 495 and mitochondrial reactive oxygen species formation in response to a high H₂O₂ concentration

Thomas Jeremy Guterbaum; Thomas Hartig Braunstein; A Fossum; N-H Holstein-Rathlou; C T Torp-Pedersen; Maria Helena Dominguez Vall-Lamora

doi:10.1159/000354225

Endothelial nitric oxide synthase phosphorylation at Threonine 495 and mitochondrial reactive oxygen species formation in response to a high H₂O₂ concentration

Thomas Jeremy Guterbaum, Thomas Hartig Braunstein, A Fossum, N-H Holstein-Rathlou, C T Torp-Pedersen, Maria Helena Dominguez Vall-Lamora

16 Citations (Scopus)

Abstract

Hydrogen peroxide (H₂O₂) is produced in vessels during ischemia/reperfusion and during inflammation, both leading to vascular dysfunction. We investigated cellular pathways involved in endothelial nitric oxide synthase (eNOS) phosphorylation at Threonine 495 (Thr(495)) in human umbilical vein endothelial cells (HUVECs) exposed to H₂O₂.

Original language	English
Journal	Journal of Vascular Research
Volume	50
Issue number	5
Pages (from-to)	410-20
Number of pages	11
ISSN	1018-1172
DOIs	https://doi.org/10.1159/000354225
Publication status	Published - 2013

Keywords

Acetylcysteine
Butadienes
Cells, Cultured
Human Umbilical Vein Endothelial Cells
Humans
Hydrogen Peroxide
Indoles
MAP Kinase Kinase Kinases
Maleimides
Mitochondria
Nitric Oxide Synthase Type III
Nitriles
Phosphorylation
Protein Kinase C
Reactive Oxygen Species
Threonine
rho-Associated Kinases

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10.1159/000354225

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Guterbaum, T. J., Braunstein, T. H., Fossum, A., Holstein-Rathlou, N.-H., Torp-Pedersen, C. T., & Dominguez Vall-Lamora, M. H. (2013). Endothelial nitric oxide synthase phosphorylation at Threonine 495 and mitochondrial reactive oxygen species formation in response to a high H₂O₂ concentration. Journal of Vascular Research, 50(5), 410-20. https://doi.org/10.1159/000354225

Endothelial nitric oxide synthase phosphorylation at Threonine 495 and mitochondrial reactive oxygen species formation in response to a high H₂O₂ concentration. / Guterbaum, Thomas Jeremy; Braunstein, Thomas Hartig; Fossum, A et al.
In: Journal of Vascular Research, Vol. 50, No. 5, 2013, p. 410-20.

Research output: Contribution to journal › Journal article › Research › peer-review

Guterbaum, TJ, Braunstein, TH, Fossum, A, Holstein-Rathlou, N-H, Torp-Pedersen, CT & Dominguez Vall-Lamora, MH 2013, 'Endothelial nitric oxide synthase phosphorylation at Threonine 495 and mitochondrial reactive oxygen species formation in response to a high H₂O₂ concentration', Journal of Vascular Research, vol. 50, no. 5, pp. 410-20. https://doi.org/10.1159/000354225

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title = "Endothelial nitric oxide synthase phosphorylation at Threonine 495 and mitochondrial reactive oxygen species formation in response to a high H₂O₂ concentration",

abstract = "Hydrogen peroxide (H₂O₂) is produced in vessels during ischemia/reperfusion and during inflammation, both leading to vascular dysfunction. We investigated cellular pathways involved in endothelial nitric oxide synthase (eNOS) phosphorylation at Threonine 495 (Thr(495)) in human umbilical vein endothelial cells (HUVECs) exposed to H₂O₂.",

keywords = "Acetylcysteine, Butadienes, Cells, Cultured, Human Umbilical Vein Endothelial Cells, Humans, Hydrogen Peroxide, Indoles, MAP Kinase Kinase Kinases, Maleimides, Mitochondria, Nitric Oxide Synthase Type III, Nitriles, Phosphorylation, Protein Kinase C, Reactive Oxygen Species, Threonine, rho-Associated Kinases",

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AU - Fossum, A

AU - Holstein-Rathlou, N-H

AU - Torp-Pedersen, C T

AU - Dominguez Vall-Lamora, Maria Helena

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AB - Hydrogen peroxide (H₂O₂) is produced in vessels during ischemia/reperfusion and during inflammation, both leading to vascular dysfunction. We investigated cellular pathways involved in endothelial nitric oxide synthase (eNOS) phosphorylation at Threonine 495 (Thr(495)) in human umbilical vein endothelial cells (HUVECs) exposed to H₂O₂.

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KW - Butadienes

KW - Cells, Cultured

KW - Human Umbilical Vein Endothelial Cells

KW - Humans

KW - Hydrogen Peroxide

KW - Indoles

KW - MAP Kinase Kinase Kinases

KW - Maleimides

KW - Mitochondria

KW - Nitric Oxide Synthase Type III

KW - Nitriles

KW - Phosphorylation

KW - Protein Kinase C

KW - Reactive Oxygen Species

KW - Threonine

KW - rho-Associated Kinases

U2 - 10.1159/000354225

DO - 10.1159/000354225

M3 - Journal article

C2 - 24008236

SN - 1018-1172

VL - 50

SP - 410

EP - 420

JO - Journal of Vascular Research

JF - Journal of Vascular Research

IS - 5

ER -

Endothelial nitric oxide synthase phosphorylation at Threonine 495 and mitochondrial reactive oxygen species formation in response to a high H₂O₂ concentration

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