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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Endothelial glycocalyx on brain endothelial cells is lost in experimental cerebral malaria

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We hypothesized that the glycocalyx, which is important for endothelial integrity, is lost in severe malaria. C57BL/6 mice were infected with Plasmodium berghei ANKA, resulting in cerebral malaria, or P. chabaudi AS, resulting in uncomplicated malaria. We visualized the glycocalyx with transmission electron microscopy and measured circulating glycosaminoglycans by dot blot and ELISA. The glycocalyx was degraded in brain vasculature in cerebral and to a lesser degree uncomplicated malaria. It was affected on both intact and apoptotic endothelial cells. Circulating glycosaminoglycan levels suggested that glycocalyx disruption preceded cerebral manifestations. The contribution of this loss to pathogenesis should be studied further.

Original languageEnglish
JournalJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
Volume34
Issue number7
Pages (from-to)1107-10
Number of pages4
ISSN0271-678X
DOIs
Publication statusPublished - Jul 2014

    Research areas

  • Animals, Disease Models, Animal, Endothelial Cells, Enzyme-Linked Immunosorbent Assay, Female, Glycocalyx, Glycosaminoglycans, Immunoblotting, Malaria, Cerebral, Mice, Mice, Inbred C57BL, Microscopy, Electron, Transmission

ID: 44829266