TY - JOUR
T1 - Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene
T2 - Be alert of false negative test results
AU - Jørgensen, Rikke Lind
AU - Lerche, Christian Johann
AU - Pedersen, Martin Schou
AU - Kirkby, Nikolai
AU - Botnen, Amanda Bolt
AU - Trebbien, Ramona
AU - Nilsson-Møller, Stephen
AU - Pinholt, Mette
AU - Nielsen, Alex Christian Yde
AU - Westh, Henrik
AU - Lisby, Jan Gorm
AU - Schneider, Uffe Vest
N1 - This article is protected by copyright. All rights reserved.
PY - 2022/10
Y1 - 2022/10
N2 - In March 2022, we observed samples with a negative fluorescent signal (60.5%, n=43) for the influenza A matrix gene, and a stronger positive signal for subtype A(H3N2). Forty-three samples were positive in InfA (H3N2) (mean Cq 30.9, range 23.9-35.1) and 26 of the 43 samples were negative in InfA matrix (mean Cq 28.0, range 23.2-30.6). Our multiplex test is a laboratory developed four-target, four-color influenza A reverse-transcription PCR assay targeting the matrix gene, subtypes A(H3N2) and A(H1N1)pdm09. Several samples were negative when retested on commercial influenza Point-of-Care assays. As the matrix gene is a stand-alone target in most commercial diagnostic assays, we caution against false negative subtype A test results.
AB - In March 2022, we observed samples with a negative fluorescent signal (60.5%, n=43) for the influenza A matrix gene, and a stronger positive signal for subtype A(H3N2). Forty-three samples were positive in InfA (H3N2) (mean Cq 30.9, range 23.9-35.1) and 26 of the 43 samples were negative in InfA matrix (mean Cq 28.0, range 23.2-30.6). Our multiplex test is a laboratory developed four-target, four-color influenza A reverse-transcription PCR assay targeting the matrix gene, subtypes A(H3N2) and A(H1N1)pdm09. Several samples were negative when retested on commercial influenza Point-of-Care assays. As the matrix gene is a stand-alone target in most commercial diagnostic assays, we caution against false negative subtype A test results.
KW - Assay
KW - H3N2
KW - M gene
KW - RT-PCR
KW - diagnostic
KW - genetic drift
KW - mutations
KW - sequencing
KW - surveillance
UR - http://www.scopus.com/inward/record.url?scp=85135608051&partnerID=8YFLogxK
U2 - 10.1111/apm.13262
DO - 10.1111/apm.13262
M3 - Comment/debate
C2 - 35836366
VL - 130
SP - 612
EP - 617
JO - APMIS - Journal of Pathology, Microbiology and Immunology
JF - APMIS - Journal of Pathology, Microbiology and Immunology
SN - 0903-4641
IS - 10
ER -