Abstract
BACKGROUND: The glucagon-like peptides (GLP) 1 and 2 are secreted postprandially from L cells located mainly in the ileum. Both hormones prolong intestinal transit and GLP-2 is intestinotrophic in rodents. Patients with a jejunostomy have poor adaptation, rapid gastric and intestinal transit, and impaired postprandial GLP-2 secretion. Ileum resected short bowel patients with a preserved colon show evidence of functional adaptation and have normal gastric emptying.
AIM: To investigate if GLP-1 and GLP-2 contribute to the positive effects of a preserved colon in short bowel patients by measuring circulating levels of GLP-1 and GLP-2 in seven ileum resected short bowel patients with a preserved colon and seven age and sex matched controls.
METHODS: GLP-1 and GLP-2 immunoreactivity was measured by specific radioimmunoassays in plasma collected at fasting and at regular intervals 180 minutes after a test meal.
RESULTS: Median (25-75%) fasting GLP-2 values were 72 (69-105) pmol/l versus 23 (19-27) pmol/l (p=0.001) and meal stimulated area under the curve was 21 078 (14 811-26 610) min x pmol/l versus 11 150 (7151-12 801) min x pmol/l (p=0.01) in short bowel patients with a preserved colon compared with control subjects. Fasting GLP-1 values were 10 (6-12) pmol/l versus 5 (3-5) pmol/l (p=0.01) and meal stimulated area under the curve was 3418 (2966-6850) min x pmol/l versus 2478 (1929-3199) min x pmol/l (p=0.04), respectively.
CONCLUSION: Ileum resected short bowel patients with a preserved colon had elevated fasting plasma concentrations of GLP-1 and GLP-2 and significantly larger meal stimulated areas under the curve compared with age and sex matched controls. Elevated GLP-1 and GLP-2 concentrations may contribute to the positive effects of a preserved colon on intestinal motility and functional adaptation in ileum resected short bowel patients.
Original language | English |
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Journal | Gut |
Volume | 47 |
Issue number | 3 |
Pages (from-to) | 370-6 |
Number of pages | 7 |
ISSN | 0017-5749 |
DOIs | |
Publication status | Published - Sept 2000 |
Externally published | Yes |
Keywords
- Adaptation, Physiological
- Adult
- Aged
- Area Under Curve
- Case-Control Studies
- Fasting/blood
- Female
- Glucagon/blood
- Glucagon-Like Peptide 1
- Glucagon-Like Peptide 2
- Humans
- Ileum/physiology
- Male
- Middle Aged
- Peptide Fragments/blood
- Peptides/blood
- Postprandial Period
- Protein Precursors/blood
- Radioimmunoassay
- Short Bowel Syndrome/blood