Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Effects of calcium source, inulin, and lactose on gut-bone associations in an ovarierectomized rat model

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Rational Design, Structure-Activity Relationship, and Immunogenicity of Hypoallergenic Pru p 3 Variants

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Resveratrol inhibits steroidogenesis in human fetal adrenocortical cells at the end of first trimester

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Circadian rhythm of markers of bone turnover in patients with chronic kidney disease

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. The Arg82Cys polymorphism of the protein Nepmucin implies a role in HDL metabolism

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Administration of whey protein complexed vitamin D3 to vitamin D3-deficient growing Sprague-Dawley rats

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Bone involvement in patients with spondyloarthropathies

    Research output: Contribution to journalReviewResearchpeer-review

  • Weiwei He
  • Zhuqing Xie
  • Rebekka Thøgersen
  • Martin Krøyer Rasmussen
  • Line F Zachariassen
  • Niklas Rye Jørgensen
  • Jan Vaerum Nørgaard
  • Henrik J Andersen
  • Dennis S Nielsen
  • Axel K Hansen
  • Hanne Christine Bertram
View graph of relations

SCOPE: Osteoporosis poses a health challenge especially for postmenopausal women. This study aims to explore nutritional strategies to counteract bone demineralization in ovarierectomized (OVX) rats.

METHODS AND RESULTS: OVX rats (n = 49) are fed with one of six different diets, where two different calcium sources (dairy calcium or calcium carbonate) are provided alone or in combination with either inulin (5%) or lactose (0.5%). In addition, a calcium-deficient diet is included. Calcium supplementation increases intestinal concentrations of short-chain fatty acids (SCFAs) and the abundance of fecal Acinetobacter and Propionibacterium. Accompanied with these effects, rats fed with calcium-fortified diets have higher bone mineral density, bone mineral content and femur mechanical strength, lower serum levels of bone markers, and lower expression of calcium absorption-related genes (transient receptor potential vanilloid type 6 (TRPV6), calcium-binding protein (CaBP) compared with control. Inulin supplementation results in a markedly increased production of intestinal SCFAs, a decreased intestinal pH, an increased abundance of Allobaculum and Bifidobacterium, and an increased expression of Trpv6. Inulin and lactose show beneficial effects on spine bone.

CONCLUSION: Calcium modulates gut microbiome composition and function. A pronounced effect of inulin on metabolic activity in the gastrointestinal tract is evident, and lactose supplementation decreases jejunal pH that might be associated with slightly enhanced bone mineralization.

Original languageEnglish
Article numbere2100883
JournalMolecular Nutrition & Food Research
Volume66
Issue number8
ISSN1613-4125
DOIs
Publication statusPublished - Apr 2022

Bibliographical note

© 2022 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH GmbH.

    Research areas

  • Animals, Bone Density, Calcium, Dietary/pharmacology, Calcium/metabolism, Fatty Acids, Volatile/metabolism, Female, Gastrointestinal Microbiome, Humans, Inulin/chemistry, Lactose/pharmacology, Rats, calcium absorption, bone mineralization, gut microbiome, gut metabolomics, prebiotics

ID: 77600283