Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Effect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injury

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Reliable and valid assessment of procedural skills in resuscitative endovascular balloon occlusion of the aorta (REBOA)

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. The Geriatric Nutritional Risk Index is a powerful predictor of adverse outcome in the elderly emergency surgery patient

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Identification of a new genetic variant associated with cholecystitis: a multicenter genome-wide association study

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Most of the variation in length of stay in emergency general surgery is not related to clinical factors of patient care

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Exploration of the induced cytokine responses in European Lyme neuroborreliosis: A longitudinal cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Endotheliopathy is associated with slower liberation from mechanical ventilation: a cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Causal inference for planning randomised critical care trials: Protocol for a scoping review

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Genetic Variants Close to TTN, NKX2-5, and MYH6 Associate With AVNRT

    Research output: Contribution to journalLetterResearchpeer-review

View graph of relations

BACKGROUND: In isolated traumatic brain injury (TBI), little is known about the endothelial response and the effects of endothelial glycocalyx shedding. We have previously shown that treatment with valproic acid (VPA) improves outcomes following TBI and hemorrhagic shock.In this model, we hypothesized that severe isolated TBI would cause shedding of the endothelial glycocalyx, as measured by serum syndecan-1 (sSDC-1) levels. We further hypothesized that VPA treatment would reduce this response and reduce lesion size volume.

METHODS: Forty Sprague-Dawley rats were allocated to TBI + VPA (n = 8), TBI + saline vehicle control infusion (n = 8), sham + saline vehicle control infusion (n = 6), or sham + VPA (n = 8). TBI animals were subjected to severe controlled cortical impact and killed 6 hours after injury. VPA 300 mg/kg was given as an intravenous bolus 30 minutes after injury. Serum samples were analyzed for sSDC-1, and lesion size was determined on Nissl-stained cryosections.

RESULTS: sSDC-1 was significantly elevated in injured compared with uninjured animals at 3 hours (p = 0.0009) and 6 hours (p = 0.0007) after injury. This effect was significantly more pronounced in the animals treated with VPA (p = 0.019) 3 hours after injury, in which sSDC-1 levels were also significantly inversely correlated with lesion size (ρ = -0.55, p = 0.038).Lesion size was significantly smaller in TBI + VPA (40.45 mm ± 13.83 mm) as compared with vehicle control (59.57 mm ± 16.83 mm) (p = 0.023).

CONCLUSION: Severe isolated TBI caused shedding of the endothelial glycocalyx. Treatment with VPA was associated with increased glycocalyx shedding and reduced lesion size volume in injured animal.

Original languageEnglish
JournalThe journal of trauma and acute care surgery
Volume77
Issue number2
Pages (from-to)292-7
Number of pages6
ISSN0022-5282
DOIs
Publication statusPublished - Aug 2014

ID: 44470588