Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Effect of TRPA1 activator allyl isothiocyanate (AITC) on rat dural and pial arteries

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Altered surface expression of P2Y11 receptor with narcolepsy-associated mutations

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Novel variant of CYP2D6*6 is undetected by a commonly used genotyping procedure

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. PACAP27 induces migraine-like attacks in migraine patients

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Calcitonin gene-related peptide - beyond migraine prophylaxis

    Research output: Contribution to journalComment/debateResearchpeer-review

  3. Proposed new diagnostic criteria for chronic migraine

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. New diagnostic criteria for headache attributed to transient ischemic attacks

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

BACKGROUND: Transient receptor potential ankyrin 1 (TRPA1) channels may have a role in migraine as some substances known to cause headache activate the channel. In the craniovascular system such activation causes a calcitonin gene-related peptide (CGRP)-dependent increase in meningeal blood flow. TRPA1 channels in the endothelium of cerebral arteries cause vasodilation when activated. The headache preventive substance feverfew inhibits activation of TRPA1 channels. In this study we aim to compare and characterize the effect of the TRPA1 agonist allyl isothiocyanate (AITC) on the diameter of rat dural and pial arteries in vivo.

METHODS: The genuine closed-cranial window technique in rats was used to examine changes in dural and pial artery diameter and mean arterial blood pressure (MABP) after intracarotid infusion of AITC. Blockade experiments were performed by intravenous infusion of olcegepant, HC-030031, sumatriptan or capsazepine immediately after infusion of AITC, in four different groups of rats.

RESULTS: AITC caused a significant dilation of dural arteries, which was inhibited by HC-030031, olcegepant and sumatriptan, but not by capsazepine. In pial arteries AITC caused a significant dilation, which was not inhibited by any of the pre-treatments, suggesting a poor penetration of the blood-brain barrier or autoregulation due to dimethyl sulfoxide (DMSO) mediated decrease in MABP during HC-030031 infusion. AITC did not cause a significant change in MABP.

CONCLUSION: AITC causes dilation of dural arteries via a mechanism dependent on CGRP and TRPA1 that is sensitive to sumatriptan. AITC causes a small but significant dilation of pial arteries.

Original languageEnglish
JournalPharmacological Reports
Volume71
Issue number4
Pages (from-to)565-572
Number of pages8
ISSN1734-1140
DOIs
Publication statusPublished - Aug 2019

ID: 58920267