Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Effect of closed v. intermittent-flow respirometry on hypoxia tolerance in the shiner perch Cymatogaster aggregata

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

APA

CBE

MLA

Vancouver

Author

Snyder, S. ; Nadler, L. E. ; Bayley, J. S. ; Svendsen, M. B.S. ; Johansen, J. L. ; Domenici, P. ; Steffensen, J. F. / Effect of closed v. intermittent-flow respirometry on hypoxia tolerance in the shiner perch Cymatogaster aggregata. In: Journal of Fish Biology. 2016 ; Vol. 88, No. 1. pp. 252-264.

Bibtex

@article{0da708c7a3b1466a8e3801e7c6902a9a,
title = "Effect of closed v. intermittent-flow respirometry on hypoxia tolerance in the shiner perch Cymatogaster aggregata",
abstract = "This study compares the critical oxygen saturation (O2 crit) levels of the shiner perch Cymatogaster aggregata obtained using two different methods wherein hypoxia is induced either by the fish's respiration (closed respirometry) or by degassing oxygen with nitrogen (intermittent-flow respirometry). Fish exhibited loss of equilibrium at a higher O2 saturation in the closed respirometry method when compared with the intermittent-flow method. Utilization of closed respirometry yielded O2 crit measurements that were almost twice as high as those obtained with intermittent-flow respirometry. The lower hypoxia tolerance in closed respirometry is consistent with additional stress, caused by a build-up of ammonia and carbon dioxide and a faster rate in dissolved oxygen decline. The results indicate that these two methods of determining hypoxia tolerance in aquatic organisms are not comparable, and that much care should be given to method choice.",
keywords = "Aquatic breathers, Critical oxygen saturation level, Hypoxic, Physiology, Respiration, Stress",
author = "S. Snyder and Nadler, {L. E.} and Bayley, {J. S.} and Svendsen, {M. B.S.} and Johansen, {J. L.} and P. Domenici and Steffensen, {J. F.}",
year = "2016",
month = "1",
day = "1",
doi = "10.1111/jfb.12837",
language = "English",
volume = "88",
pages = "252--264",
journal = "Journal of Fish Biology",
issn = "0022-1112",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Effect of closed v. intermittent-flow respirometry on hypoxia tolerance in the shiner perch Cymatogaster aggregata

AU - Snyder, S.

AU - Nadler, L. E.

AU - Bayley, J. S.

AU - Svendsen, M. B.S.

AU - Johansen, J. L.

AU - Domenici, P.

AU - Steffensen, J. F.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - This study compares the critical oxygen saturation (O2 crit) levels of the shiner perch Cymatogaster aggregata obtained using two different methods wherein hypoxia is induced either by the fish's respiration (closed respirometry) or by degassing oxygen with nitrogen (intermittent-flow respirometry). Fish exhibited loss of equilibrium at a higher O2 saturation in the closed respirometry method when compared with the intermittent-flow method. Utilization of closed respirometry yielded O2 crit measurements that were almost twice as high as those obtained with intermittent-flow respirometry. The lower hypoxia tolerance in closed respirometry is consistent with additional stress, caused by a build-up of ammonia and carbon dioxide and a faster rate in dissolved oxygen decline. The results indicate that these two methods of determining hypoxia tolerance in aquatic organisms are not comparable, and that much care should be given to method choice.

AB - This study compares the critical oxygen saturation (O2 crit) levels of the shiner perch Cymatogaster aggregata obtained using two different methods wherein hypoxia is induced either by the fish's respiration (closed respirometry) or by degassing oxygen with nitrogen (intermittent-flow respirometry). Fish exhibited loss of equilibrium at a higher O2 saturation in the closed respirometry method when compared with the intermittent-flow method. Utilization of closed respirometry yielded O2 crit measurements that were almost twice as high as those obtained with intermittent-flow respirometry. The lower hypoxia tolerance in closed respirometry is consistent with additional stress, caused by a build-up of ammonia and carbon dioxide and a faster rate in dissolved oxygen decline. The results indicate that these two methods of determining hypoxia tolerance in aquatic organisms are not comparable, and that much care should be given to method choice.

KW - Aquatic breathers

KW - Critical oxygen saturation level

KW - Hypoxic

KW - Physiology

KW - Respiration

KW - Stress

UR - http://www.scopus.com/inward/record.url?scp=84956739417&partnerID=8YFLogxK

U2 - 10.1111/jfb.12837

DO - 10.1111/jfb.12837

M3 - Journal article

VL - 88

SP - 252

EP - 264

JO - Journal of Fish Biology

JF - Journal of Fish Biology

SN - 0022-1112

IS - 1

ER -

ID: 56055467