TY - JOUR
T1 - Dried blood spot
T2 - A diagnostic detection method for HBV, HCV and HIV nucleic acid using a single drop of blood
AU - Nilsson, Stephen Strunge
AU - Demant, Jonas
AU - Thønnings, Sara
AU - Weis, Nina
AU - Westh, Henrik
AU - Pinholt, Mette
N1 - Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.
PY - 2025/3
Y1 - 2025/3
N2 - The global strategy to eradicate Hepatitis B (HBV), Hepatitis C (HCV), and HIV by 2030 is critical due to their impact and challenges to healthcare systems. HCV is curable, but HBV and HIV are only suppressible, with a vaccine available solely for HBV. Innovative diagnostic methods are needed, especially for high-risk populations like people who inject drugs (PWID). This study validates a dried blood spot (DBS) nucleic acid amplification test (NAAT) using the Hologic Panther system for detecting HBV, HCV, and HIV. The method was used to screen among PWID in the Capital Region of Denmark. The DBS method demonstrated high sensitivity, with a 95 % limit of detection (LoD) of 2711 IU/mL for HBV, 525 IU/mL for HCV, and 4022 copies/mL for HIV. Screening of 83 PWID in Denmark revealed a 13 % prevalence of active HCV infection, offering significant benefits in settings where traditional venous access is difficult.
AB - The global strategy to eradicate Hepatitis B (HBV), Hepatitis C (HCV), and HIV by 2030 is critical due to their impact and challenges to healthcare systems. HCV is curable, but HBV and HIV are only suppressible, with a vaccine available solely for HBV. Innovative diagnostic methods are needed, especially for high-risk populations like people who inject drugs (PWID). This study validates a dried blood spot (DBS) nucleic acid amplification test (NAAT) using the Hologic Panther system for detecting HBV, HCV, and HIV. The method was used to screen among PWID in the Capital Region of Denmark. The DBS method demonstrated high sensitivity, with a 95 % limit of detection (LoD) of 2711 IU/mL for HBV, 525 IU/mL for HCV, and 4022 copies/mL for HIV. Screening of 83 PWID in Denmark revealed a 13 % prevalence of active HCV infection, offering significant benefits in settings where traditional venous access is difficult.
KW - Dried blood spot
KW - Hepatitis B
KW - Hepatitis C
KW - HIV
KW - People who inject drugs
KW - Viral load
UR - http://www.scopus.com/inward/record.url?scp=85212432924&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2024.116661
DO - 10.1016/j.diagmicrobio.2024.116661
M3 - Journal article
C2 - 39706101
SN - 0732-8893
VL - 111
SP - 116661
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 3
M1 - 116661
ER -