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Dissemination and Characteristics of a Novel Plasmid-Encoded Carbapenem-Hydrolyzing Class D β-Lactamase, OXA-436 from Four Patients Involving Six Different Hospitals in Denmark

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  • Ørjan Samuelsen
  • Frank Hansen
  • Bettina Aasnæs
  • Henrik Hasman
  • Bjarte Aarmo Lund
  • Hanna-Kirsti S Leiros
  • Berit Lilje
  • Jessin Janice
  • Lotte Jakobsen
  • Pia Littauer
  • Lillian M Søes
  • Barbara J Holzknecht
  • Leif P Andersen
  • Marc Stegger
  • Paal S Andersen
  • Anette M Hammerum
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The diversity of OXA-48-like carbapenemases is continually expanding. In this study, we describe the dissemination and characteristics of a novel carbapenem-hydrolyzing class D carbapenemase (CHDL) named OXA-436. In total, six OXA-436-producing Enterobacteriaceae isolates including Enterobacter asburiae (n=3), Citrobacter freundii (n=2) and Klebsiella pneumoniae (n=1) were identified in four patients in the period between September 2013 and April 2015. All three species of OXA-436-producing Enterobacteriaceae were found in one patient. The amino acid sequence of OXA-436 showed 90.4-92.8% identity to other acquired OXA-48-like variants. Expression of OXA-436 in Escherichia coli and kinetic analysis of purified OXA-436 revealed an activity profile similar to OXA-48 and OXA-181 with activity against penicillins including temocillin, limited or no activity against extended-spectrum cephalosporins and activity against carbapenems. The blaOXA-436 gene was located on a conjugative ∼314 kb IncHI2/IncHI2A plasmid belonging to pMLST ST1, in a region surrounded by chromosomal genes previously identified adjacent to blaOXA-genes in Shewanella spp. In conclusion, OXA-436 is a novel CHDL with similar functional properties as OXA-48-like CHDLs. The described geographical spread among different Enterobacteriaceae and plasmid location of blaOXA-436 illustrates its potential for further dissemination.

Original languageEnglish
JournalAntimicrobial Agents and Chemotherapy
Issue number1
Pages (from-to)e01260-17
Publication statusPublished - 2018

    Research areas

  • Journal Article

ID: 51951755