Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

Tine Y Wolff; Steffen Eickhardt; Maria Kristin Björnsdottir; Claus Moser; Thomas Bjarnsholt; Niels Høiby; Trine Rolighed Thomsen

doi:10.1128/JCM.00190-13

Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

Tine Y Wolff, Steffen Eickhardt, Maria Kristin Björnsdottir, Claus Moser, Thomas Bjarnsholt, Niels Høiby, Trine Rolighed Thomsen

Department of Clinical Microbiology

7 Citations (Scopus)

Abstract

In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

Original language	English
Journal	Journal of Clinical Microbiology
Volume	51
Issue number	4
Pages (from-to)	1281-4
Number of pages	4
ISSN	0095-1137
DOIs	https://doi.org/10.1128/JCM.00190-13
Publication status	Published - Apr 2013

Keywords

Adult
Aged
Bacterial Infections
Coinfection
Computational Biology
Female
Humans
Male
Middle Aged
Molecular Diagnostic Techniques
Sequence Analysis, DNA
Software

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10.1128/JCM.00190-13

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abstract = "In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.",

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year = "2013",

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T1 - Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

AU - Wolff, Tine Y

AU - Eickhardt, Steffen

AU - Björnsdottir, Maria Kristin

AU - Moser, Claus

AU - Bjarnsholt, Thomas

AU - Høiby, Niels

AU - Thomsen, Trine Rolighed

PY - 2013/4

Y1 - 2013/4

N2 - In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

AB - In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

KW - Adult

KW - Aged

KW - Bacterial Infections

KW - Coinfection

KW - Computational Biology

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Molecular Diagnostic Techniques

KW - Sequence Analysis, DNA

KW - Software

UR - https://www.scopus.com/pages/publications/84875818919

U2 - 10.1128/JCM.00190-13

DO - 10.1128/JCM.00190-13

M3 - Journal article

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SN - 0095-1137

VL - 51

SP - 1281

EP - 1284

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 4

ER -

Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

Abstract

Keywords

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