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Different mechanisms are involved in the antibody mediated inhibition of ligand binding to the urokinase receptor: a study based on biosensor technology

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  1. An IgY-based immunoassay to evaluate the biomarker potential of the Tannerella forsythia virulence factor karilysin in human saliva

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  2. The use of synthetic peptides for detection of anti-citrullinated protein antibodies in rheumatoid arthritis

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  3. A novel antihuman C3d monoclonal antibody with specificity to the C3d complement split product

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  4. Efficient evaluation of humoral immune responses by the use of serum pools

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  1. Tumor cell MT1-MMP is dispensable for osteosarcoma tumor growth, bone degradation and lung metastasis

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  2. The collagen receptor uPARAP/Endo180 regulates collectins through unique structural elements in its FNII domain

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  3. Cellular uptake of collagens and implications for immune cell regulation in disease

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  4. TAFI deficiency causes maladaptive vascular remodeling after hemophilic joint bleeding

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Certain monoclonal antibodies are capable of inhibiting the biological binding reactions of their target proteins. At the molecular level, this type of effect may be brought about by completely different mechanisms, such as competition for common binding determinants, steric hindrance or interference with conformational properties of the receptor critical for ligand binding. This distinction is central when employing the antibodies as tools in the elucidation of the structure-function relationship of the protein in question. We have studied the effect of monoclonal antibodies against the urokinase plasminogen activator receptor (uPAR), a protein located on the surface of various types of malignant and normal cells which is involved in the direction of proteolytic degradation reactions in the extracellular matrix. We show that surface plasmon resonance/biomolecular interaction analysis (BIA) can be employed as a highly useful tool to characterize the inhibitory mechanism of specific antagonist antibodies. Two inhibitory antibodies against uPAR, mAb R3 and mAb R5, were shown to exhibit competitive and non-competitive inhibition, respectively, of ligand binding to the receptor. The former antibody efficiently blocked the receptor against subsequent ligand binding but was unable to promote the dissociation of a preformed receptor-ligand complex. The latter antibody was capable of binding the preformed complex, forming a transient trimolecular assembly, and promoting the dissociation of the uPA/uPAR complex. The continuous recording of binding and dissociation, obtained in BIA, is central in characterizing these phenomena. The identification of a non-competitive inhibitory mechanism against this receptor reveals the presence of a determinant which influences the binding properties of a remote site in the molecular structure and which could be an important target for a putative synthetic antagonist.

Original languageEnglish
JournalJournal of Immunological Methods
Volume222
Issue number1-2
Pages (from-to)125-33
Number of pages9
ISSN0022-1759
Publication statusPublished - 1 Jan 1999

    Research areas

  • Animals, Antibodies, Monoclonal, Biosensing Techniques, CHO Cells, Cricetinae, Humans, Iodine Radioisotopes, Ligands, Peptide Fragments, Receptors, Cell Surface, Receptors, Urokinase Plasminogen Activator, Recombinant Proteins, Solubility, Transfection, Urokinase-Type Plasminogen Activator

ID: 46435314