Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Differences in Cell Cycle Status Underlie Transcriptional Heterogeneity in the HSC Compartment

Research output: Contribution to journalJournal articleResearchpeer-review

  1. ERG Controls B Cell Development by Promoting Igh V-to-DJ Recombination

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Single mRNP Analysis Reveals that Small Cytoplasmic mRNP Granules Represent mRNA Singletons

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. De Novo Sequence and Copy Number Variants Are Strongly Associated with Tourette Disorder and Implicate Cell Polarity in Pathogenesis

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Targeted inhibition of cooperative mutation- and therapy-induced AKT activation in AML effectively enhances response to chemotherapy

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Quantitative single-cell proteomics as a tool to characterize cellular hierarchies

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Basement membrane stiffness determines metastases formation

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. The ASXL1-G643W variant accelerates the development of CEBPA mutant acute myeloid leukemia

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Hematopoietic stem cells (HSCs) are considered a heterogeneous cell population. To further resolve the HSC compartment, we characterized a retinoic acid (RA) reporter mouse line. Sub-fractionation of the HSC compartment in RA-CFP reporter mice demonstrated that RA-CFP-dim HSCs were largely non-proliferative and displayed superior engraftment potential in comparison with RA-CFP-bright HSCs. Gene expression analysis demonstrated higher expression of RA-target genes in RA-CFP-dim HSCs, in contrast to the RA-CFP reporter expression, but both RA-CFP-dim and RA-CFP-bright HSCs responded efficiently to RA in vitro. Single-cell RNA sequencing (RNA-seq) of >1,200 HSCs showed that differences in cell cycle activity constituted the main driver of transcriptional heterogeneity in HSCs. Moreover, further analysis of the single-cell RNA-seq data revealed that stochastic low-level expression of distinct lineage-affiliated transcriptional programs is a common feature of HSCs. Collectively, this work demonstrates the utility of the RA-CFP reporter line as a tool for the isolation of superior HSCs.

Original languageEnglish
JournalCell Reports
Volume24
Issue number3
Pages (from-to)766-780
Number of pages15
DOIs
Publication statusPublished - 17 Jul 2018

ID: 54960009