Developmental expression of the gastrin and cholecystokinin genes in rat colon

H R Lüttichau; W W Van Solinge; F C Nielsen; J F Rehfeld

doi:10.1016/0016-5085(93)90278-k

Developmental expression of the gastrin and cholecystokinin genes in rat colon

H R Lüttichau, W W Van Solinge, F C Nielsen, J F Rehfeld

61 Citations (Scopus)

Abstract

BACKGROUND: To elucidate the hypothesis that gastrin and cholecystokinin (CCK) are local growth factors for colorectal mucosa, we have examined the peptide gene expression in rat colon during development.

METHODS: Northern analysis, reverse transcription PCR, and sequence-specific radioimmunoassays were the essential methods.

RESULTS: High concentrations of gastrin and CCK messenger RNA were found in the fetal colon. At birth, gastrin and CCK mRNA's were both undetectable but increased subsequently towards adult life. The fetal colon contained 5.5 and 4.2 pmol/g tissue gastrin and CCK, respectively. After birth, carboxyamidated gastrin disappeared from the colon, whereas the concentration of CCK remained at 1 pmol/g. Glycine-extended gastrin and CCK were also present in the fetal colon, but towards adult life they decreased below 0.2 pmol/g. In contrast, progastrin and proCCK were detectable at all ages.

CONCLUSIONS: Rat colon expresses the gastrin and CCK genes throughout life. The posttranslational maturation of progastrin, however, ceases shortly after birth, indicating that gastrin may play a role in the developing colon. Whether CCK influences the development remains to be shown.

Original language	English
Journal	Gastroenterology
Volume	104
Issue number	4
Pages (from-to)	1092-8
Number of pages	7
ISSN	0016-5085
DOIs	https://doi.org/10.1016/0016-5085(93)90278-k
Publication status	Published - Apr 1993
Externally published	Yes

Keywords

Aging/genetics
Animals
Animals, Newborn
Blotting, Northern
Cholecystokinin/genetics
Colon/growth & development
Female
Fetus
Gastrins/genetics
Gene Expression Regulation
Oligodeoxyribonucleotides
Polymerase Chain Reaction/methods
Pregnancy
Protein Precursors/metabolism
Protein Processing, Post-Translational
RNA Probes
RNA, Messenger/analysis
Rats
Rats, Wistar
Transcription, Genetic

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10.1016/0016-5085(93)90278-k

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title = "Developmental expression of the gastrin and cholecystokinin genes in rat colon",

abstract = "BACKGROUND: To elucidate the hypothesis that gastrin and cholecystokinin (CCK) are local growth factors for colorectal mucosa, we have examined the peptide gene expression in rat colon during development.METHODS: Northern analysis, reverse transcription PCR, and sequence-specific radioimmunoassays were the essential methods.RESULTS: High concentrations of gastrin and CCK messenger RNA were found in the fetal colon. At birth, gastrin and CCK mRNA's were both undetectable but increased subsequently towards adult life. The fetal colon contained 5.5 and 4.2 pmol/g tissue gastrin and CCK, respectively. After birth, carboxyamidated gastrin disappeared from the colon, whereas the concentration of CCK remained at 1 pmol/g. Glycine-extended gastrin and CCK were also present in the fetal colon, but towards adult life they decreased below 0.2 pmol/g. In contrast, progastrin and proCCK were detectable at all ages.CONCLUSIONS: Rat colon expresses the gastrin and CCK genes throughout life. The posttranslational maturation of progastrin, however, ceases shortly after birth, indicating that gastrin may play a role in the developing colon. Whether CCK influences the development remains to be shown.",

keywords = "Aging/genetics, Animals, Animals, Newborn, Blotting, Northern, Cholecystokinin/genetics, Colon/growth \& development, Female, Fetus, Gastrins/genetics, Gene Expression Regulation, Oligodeoxyribonucleotides, Polymerase Chain Reaction/methods, Pregnancy, Protein Precursors/metabolism, Protein Processing, Post-Translational, RNA Probes, RNA, Messenger/analysis, Rats, Rats, Wistar, Transcription, Genetic",

author = "L{\"u}ttichau, \{H R\} and \{Van Solinge\}, \{W W\} and Nielsen, \{F C\} and Rehfeld, \{J F\}",

year = "1993",

month = apr,

doi = "10.1016/0016-5085(93)90278-k",

language = "English",

volume = "104",

pages = "1092--8",

journal = "Gastroenterology",

issn = "0016-5085",

publisher = "W.B. Saunders",

number = "4",

}

TY - JOUR

T1 - Developmental expression of the gastrin and cholecystokinin genes in rat colon

AU - Lüttichau, H R

AU - Van Solinge, W W

AU - Nielsen, F C

AU - Rehfeld, J F

PY - 1993/4

Y1 - 1993/4

N2 - BACKGROUND: To elucidate the hypothesis that gastrin and cholecystokinin (CCK) are local growth factors for colorectal mucosa, we have examined the peptide gene expression in rat colon during development.METHODS: Northern analysis, reverse transcription PCR, and sequence-specific radioimmunoassays were the essential methods.RESULTS: High concentrations of gastrin and CCK messenger RNA were found in the fetal colon. At birth, gastrin and CCK mRNA's were both undetectable but increased subsequently towards adult life. The fetal colon contained 5.5 and 4.2 pmol/g tissue gastrin and CCK, respectively. After birth, carboxyamidated gastrin disappeared from the colon, whereas the concentration of CCK remained at 1 pmol/g. Glycine-extended gastrin and CCK were also present in the fetal colon, but towards adult life they decreased below 0.2 pmol/g. In contrast, progastrin and proCCK were detectable at all ages.CONCLUSIONS: Rat colon expresses the gastrin and CCK genes throughout life. The posttranslational maturation of progastrin, however, ceases shortly after birth, indicating that gastrin may play a role in the developing colon. Whether CCK influences the development remains to be shown.

AB - BACKGROUND: To elucidate the hypothesis that gastrin and cholecystokinin (CCK) are local growth factors for colorectal mucosa, we have examined the peptide gene expression in rat colon during development.METHODS: Northern analysis, reverse transcription PCR, and sequence-specific radioimmunoassays were the essential methods.RESULTS: High concentrations of gastrin and CCK messenger RNA were found in the fetal colon. At birth, gastrin and CCK mRNA's were both undetectable but increased subsequently towards adult life. The fetal colon contained 5.5 and 4.2 pmol/g tissue gastrin and CCK, respectively. After birth, carboxyamidated gastrin disappeared from the colon, whereas the concentration of CCK remained at 1 pmol/g. Glycine-extended gastrin and CCK were also present in the fetal colon, but towards adult life they decreased below 0.2 pmol/g. In contrast, progastrin and proCCK were detectable at all ages.CONCLUSIONS: Rat colon expresses the gastrin and CCK genes throughout life. The posttranslational maturation of progastrin, however, ceases shortly after birth, indicating that gastrin may play a role in the developing colon. Whether CCK influences the development remains to be shown.

KW - Aging/genetics

KW - Animals

KW - Animals, Newborn

KW - Blotting, Northern

KW - Cholecystokinin/genetics

KW - Colon/growth & development

KW - Female

KW - Fetus

KW - Gastrins/genetics

KW - Gene Expression Regulation

KW - Oligodeoxyribonucleotides

KW - Polymerase Chain Reaction/methods

KW - Pregnancy

KW - Protein Precursors/metabolism

KW - Protein Processing, Post-Translational

KW - RNA Probes

KW - RNA, Messenger/analysis

KW - Rats

KW - Rats, Wistar

KW - Transcription, Genetic

UR - https://www.scopus.com/pages/publications/0027514212

U2 - 10.1016/0016-5085(93)90278-k

DO - 10.1016/0016-5085(93)90278-k

M3 - Journal article

C2 - 8462797

SN - 0016-5085

VL - 104

SP - 1092

EP - 1098

JO - Gastroenterology

JF - Gastroenterology

IS - 4

ER -

Developmental expression of the gastrin and cholecystokinin genes in rat colon

Abstract

Keywords

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