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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Development of a fast method for direct analysis of intact synthetic insulins in human plasma: the large peptide challenge

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  5. First example of hepatocyte transplantation to alleviate ornithine transcarbamylase deficiency, monitored by NMR-based metabonomics

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  1. Abnormal brain cholesterol homeostasis in Alzheimer’s disease—a targeted metabolomic and transcriptomic study

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  2. Distinct Molecular Signatures of Clinical Clusters in People with Type 2 Diabetes: an IMI-RHAPSODY Study

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  3. Essential Branched-Chain Amino Acids and Ribonic Acid Are Associated with Cardiorenal Events in Type 1 Diabetes

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  4. Prognostic performance of 7 biomarkers compared to liver biopsy in early alcohol-related liver disease

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BACKGROUND: Intact insulins are difficult to analyze by LC-MS/MS due to nonspecific binding and poor sensitivity, solubility and fragmentation. This work aims to provide a simpler, faster LC-MS method and focuses on solving the above issues.

RESULTS: A novel charged-surface chromatographic column produced peak widths for insulin that were significantly narrower than traditional C18 columns when using formic acid as mobile phase. Mass spectral fragments m/z >700 provided greater specificity, significantly reducing endogenous background. Detection limits in human plasma were 0.2 ng/ml for insulin glargine, glulisine and detemir, and 0.5 ng/ml for insulin aspart. Average accuracy for standard curve and QC samples was 93.4%.

CONCLUSION: A simple SPE LC-MS analysis was developed for direct, simultaneous quantification of insulin glargine, detemir, aspart and glulisine.

Original languageEnglish
JournalBioanalysis
Volume5
Issue number1
Pages (from-to)65-81
Number of pages17
ISSN1757-6180
DOIs
Publication statusPublished - Jan 2013
Externally publishedYes

    Research areas

  • Amino Acid Sequence, Analytic Sample Preparation Methods, Blood Chemical Analysis/methods, Calibration, Chromatography, Liquid, Humans, Insulins/blood, Limit of Detection, Mass Screening, Molecular Sequence Data, Molecular Weight, Quality Control, Surface Plasmon Resonance, Time Factors

ID: 54961156