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Detection and kinetics of persistent neutralizing anti-interferon-beta antibodies in patients with multiple sclerosis. Results from the ABIRISK prospective cohort study

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  • Poul Erik H Jensen
  • Clemens Warnke
  • Kathleen Ingenhoven
  • Luca Piccoli
  • Marcia Gasis
  • Christina Hermanrud
  • Blanca M Fernandez-Rodriguez
  • Malin Ryner
  • Daniel Kramer
  • Jenny Link
  • Ryan Ramanujam
  • Michael Auer
  • Dorothea Buck
  • Verena Grummel
  • Elisa Bertotti
  • Nicolas Fissolo
  • Begoña Oliver-Martos
  • Petra Nytrova
  • Michael Khalil
  • Michael Guger
  • Sandra Rathmaier
  • Claudia Sievers-Stober
  • Raija L P Lindberg
  • Signe Hässler
  • Delphine Bachelet
  • Orhan Aktas
  • Naoimh Donnellan
  • Andy Lawton
  • Bernhard Hemmer
  • Eva Kubala Havrdova
  • Bernd Kieseier
  • Hans-Peter Hartung
  • Manuel Comabella
  • Xavier Montalban
  • Tobias Derfuss
  • Finn Sellebjerg
  • Pierre Dönnes
  • Marc Pallardy
  • Sebastian Spindeldreher
  • Philippe Broët
  • Florian Deisenhammer
  • Anna Fogdell-Hahn
  • Per Soelberg Sorensen
  • ABIRISK Consortium
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Two validated assays, a bridging ELISA and a luciferase-based bioassay, were compared for detection of anti-drug antibodies (ADA) against interferon-beta (IFN-β) in patients with multiple sclerosis. Serum samples were tested from patients enrolled in a prospective study of 18 months. In contrast to the ELISA, when IFN-β-specific rabbit polyclonal and human monoclonal antibodies were tested, the bioassay was the more sensitive to detect IFN-β ADA in patients' sera. For clinical samples, selection of method of ELISA should be evaluated prior to the use of a multi-tiered approach. A titer threshold value is reported that may be used as a predictor for persistently positive neutralizing ADA.

Original languageEnglish
JournalJournal of Neuroimmunology
Volume326
Pages (from-to)19-27
Number of pages9
ISSN0165-5728
DOIs
Publication statusPublished - 15 Jan 2019

    Research areas

  • Antibodies, Neutralizing/blood, Biological Assay, Enzyme-Linked Immunosorbent Assay, Female, Humans, Immunologic Factors/immunology, Interferon-beta/immunology, Male, Multiple Sclerosis/blood, Neutralization Tests/methods

ID: 58577830