Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Design and setup of intermittent-flow respirometry system for aquatic organisms

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Plasma osmolality and oxygen consumption of perch Perca fluviatilis in response to different salinities and temperatures

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Effect of closed v. intermittent-flow respirometry on hypoxia tolerance in the shiner perch Cymatogaster aggregata

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Computerized feedback during colonoscopy training leads to improved performance: a randomized trial

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Methods for certification in colonoscopy - a systematic review

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. An automatic measure of progression during colonoscopy correlates to patient experienced pain

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Intermittent-flow respirometry is an experimental protocol for measuring oxygen consumption in aquatic organisms that utilizes the best features of closed (stop-flow) and flow-through respirometry while eliminating (or at least reducing) some of their inherent problems. By interspersing short periods of closed-chamber oxygen consumption measurements with regular flush periods, accurate oxygen uptake rate measurements can be made without the accumulation of waste products, particularly carbon dioxide, which may confound results. Automating the procedure with easily available hardware and software further reduces error by allowing many measurements to be made over long periods thereby minimizing animal stress due to acclimation issues. This paper describes some of the fundamental principles that need to be considered when designing and carrying out automated intermittent-flow respirometry (e.g. chamber size, flush rate, flush time, chamber mixing, measurement periods and temperature control). Finally, recent advances in oxygen probe technology and open source automation software will be discussed in the context of assembling relatively low cost and reliable measurement systems.

Original languageEnglish
JournalJournal of Fish Biology
Volume88
Issue number1
Pages (from-to)26-50
Number of pages25
ISSN0022-1112
DOIs
Publication statusPublished - 1 Jan 2016

    Research areas

  • Automated respirometry, Metabolism, Oxygen consumption

ID: 56055289