TY - JOUR
T1 - Deep immune cell phenotyping and induced immune cell responses at admission stratified by BMI in patients hospitalized with COVID-19
T2 - An observational multicenter cohort pilot study
AU - Sejdic, Adin
AU - Hartling, Hans Jakob
AU - Holler, Jon G
AU - Gjærde, Lars Klingen
AU - Dungu, Arnold Matovu
AU - Møller, Maria Elizabeth Engel
AU - Teglgaard, Rebecca Svanberg
AU - Niemann, Carsten Utoft
AU - Brooks, Patrick Terrence
AU - Mogensen, Trine H
AU - Weis, Nina
AU - Podlekareva, Daria
AU - Jørgensen, Marie Louise Baum
AU - Gang, Anne Ortved
AU - Hersby, Ditte Stampe
AU - Hald, Annemette
AU - Nielsen, Susanne Dam
AU - Lebech, Anne-Mette
AU - Helleberg, Marie
AU - Lundgren, Jens
AU - Franck, Kristina Træholt
AU - Fischer, Thea K
AU - Harboe, Zitta Barrella
AU - Marquart, Hanne Vibeke
AU - Ostrowski, Sisse Rye
AU - Lindegaard, Birgitte
N1 - Copyright © 2024. Published by Elsevier Inc.
PY - 2024/8/6
Y1 - 2024/8/6
N2 - INTRODUCTION: Overweight and obesity are linked to increased hospitalization and mortality in COVID-19 patients. This study aimed to characterize induced immune responses and deep immune cell profiles stratified by BMI in hospitalized COVID-19 patients.METHODS AND RESULTS: This observational multicenter cohort pilot study included 122 adult patients with PCR-confirmed COVID-19 in Denmark, stratified by BMI (normal weight, overweight, obese). Inflammation was assessed using TruCulture® and immune cell profiles by flow cytometry with a customized antibody panel (DuraClone®). Patients with obesity had a more pro-inflammatory phenotype with increased TNF-α, IL-8, IL-17, and IL-10 levels post-T cell stimulation, and altered B cell profiles. Patients with obesity showed higher concentrations of naïve, transitional, and non-isotype switched memory B cells, and plasmablasts compared to normal weight patients and healthy controls.CONCLUSIONS: Obesity in hospitalized COVID-19 patients may correlate with elevated pro-inflammatory cytokines, anti-inflammatory IL-10, and increased B cell subset activation, highlighting the need for further studies.
AB - INTRODUCTION: Overweight and obesity are linked to increased hospitalization and mortality in COVID-19 patients. This study aimed to characterize induced immune responses and deep immune cell profiles stratified by BMI in hospitalized COVID-19 patients.METHODS AND RESULTS: This observational multicenter cohort pilot study included 122 adult patients with PCR-confirmed COVID-19 in Denmark, stratified by BMI (normal weight, overweight, obese). Inflammation was assessed using TruCulture® and immune cell profiles by flow cytometry with a customized antibody panel (DuraClone®). Patients with obesity had a more pro-inflammatory phenotype with increased TNF-α, IL-8, IL-17, and IL-10 levels post-T cell stimulation, and altered B cell profiles. Patients with obesity showed higher concentrations of naïve, transitional, and non-isotype switched memory B cells, and plasmablasts compared to normal weight patients and healthy controls.CONCLUSIONS: Obesity in hospitalized COVID-19 patients may correlate with elevated pro-inflammatory cytokines, anti-inflammatory IL-10, and increased B cell subset activation, highlighting the need for further studies.
KW - Adaptive immunity
KW - B cells
KW - BMI
KW - COVID-19
KW - Cytokines
KW - Inflammation
KW - Innate immunity
KW - Obesity
KW - SARS-CoV-2
KW - T cells
UR - http://www.scopus.com/inward/record.url?scp=85201002798&partnerID=8YFLogxK
M3 - Journal article
C2 - 39117044
SN - 1521-6616
VL - 267
SP - 110336
JO - Clinical immunology (Orlando, Fla.)
JF - Clinical immunology (Orlando, Fla.)
M1 - 110336
ER -