Cytochemical and Histochemical Staining with Peptide Antibodies

Tina Friis; Klaus Boberg Pedersen; David Hougaard; Gunnar Houen

doi:10.1007/978-1-0716-3914-6_20

Cytochemical and Histochemical Staining with Peptide Antibodies

Tina Friis, Klaus Boberg Pedersen, David Hougaard, Gunnar Houen^*

^*Corresponding author for this work

Abstract

Peptide antibodies are particularly useful for immunocytochemistry (ICC) and immunohistochemistry (IHC), where antigens may denature due to fixation of tissues and cells. Peptide antibodies can be made to any defined sequence, including unknown putative proteins and posttranslationally modified sequences. Moreover, the availability of large amounts of the antigen (peptide) allows inhibition/absorption controls, which are important in ICC/IHC, due to the many possibilities for false-positive reactions caused by immunoglobulin Fc receptors, nonspecific reactions and cross-reactivity of primary and secondary antibodies with other antigens and endogenous immunoglobulins, respectively. Here, simple protocols for ICC and IHC are described together with recommendations for appropriate controls.

Original language	English
Title of host publication	Peptide Antibodies
Number of pages	15
Volume	2821
Publisher	Springer
Publication date	2024
Pages	249-263
DOIs	https://doi.org/10.1007/978-1-0716-3914-6_20
Publication status	Published - 2024

Series	Methods in Molecular Biology
ISSN	1064-3745

Keywords

Immunohistochemistry/methods
Staining and Labeling/methods
Antibodies/immunology
Peptides/immunology
Humans
Animals

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10.1007/978-1-0716-3914-6_20

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abstract = "Peptide antibodies are particularly useful for immunocytochemistry (ICC) and immunohistochemistry (IHC), where antigens may denature due to fixation of tissues and cells. Peptide antibodies can be made to any defined sequence, including unknown putative proteins and posttranslationally modified sequences. Moreover, the availability of large amounts of the antigen (peptide) allows inhibition/absorption controls, which are important in ICC/IHC, due to the many possibilities for false-positive reactions caused by immunoglobulin Fc receptors, nonspecific reactions and cross-reactivity of primary and secondary antibodies with other antigens and endogenous immunoglobulins, respectively. Here, simple protocols for ICC and IHC are described together with recommendations for appropriate controls.",

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AU - Friis, Tina

AU - Pedersen, Klaus Boberg

AU - Hougaard, David

AU - Houen, Gunnar

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Y1 - 2024

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KW - Staining and Labeling/methods

KW - Antibodies/immunology

KW - Peptides/immunology

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KW - Animals

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Cytochemical and Histochemical Staining with Peptide Antibodies

Abstract

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