Abstract
Antigenic analysis of M. paratuberculosis revealed extensive cross-reactivity with M. avium; however, the number of cross-reactive antigens found was dependent on the strain of M. avium tested. One antigen was shown to be the common antigen while another appeared to be iron-regulated in its production. A commercial polyclonal antibody to M. paratuberculosis produced a CIE precipitin pattern comparable to that of the antibody produced for the present study. An antigen designated no. 6 was consistently precipitated by sera from cattle infected with M. paratuberculosis. This antigen exhibited complete cross-reaction with M. avium and partial cross-reaction with M. phlei. Among three commercially available complement fixation (CF) antigen that could be precipitated by M. paratuberculosis antibodies. A commercial antigen for use in an agar gel immunodiffusion test for Johne's disease diagnosis produced 12 precipitins with the M. paratuberculosis antibody, one of which was identical with antigen 6.
| Original language | English |
|---|---|
| Journal | APMIS - Journal of Pathology, Microbiology and Immunology |
| Volume | 99 |
| Issue number | 1 |
| Pages (from-to) | 83-92 |
| Number of pages | 10 |
| ISSN | 0903-4641 |
| Publication status | Published - Jan 1991 |
| Externally published | Yes |
Keywords
- Animals
- Antibodies, Bacterial/immunology
- Antigens, Bacterial/analysis
- Cattle
- Cross Reactions
- Immunoelectrophoresis, Two-Dimensional
- Mycobacterium/immunology
- Paratuberculosis/microbiology
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