Abstract
Forty-one different antigens were demonstrated in an antigen preparation obtained by sonication of a Haemophilus influenzae (H. influenzae) type b strain, using crossed immunoelectrophoresis and antiserum obtained from rabbits. Antigens were characterized by absorption experiments with whole heat-killed bacteria, temperature resistance and protein and polysaccharide staining. Cross-reactions between H. influenzae type b and 19 other bacterial species were studied by various quantitative immunoelectrophoretic methods, using the reference system. A non-capsulated H. influenzae cross-reacted extensively (41 antigens) with H. influenzae type b and Haemophilus parainfluenzae, and Haemophilus haemolyticus showed considerable cross-reactivity with H. influenzae type b (26 and 32 antigens respectively), while antigens from eight other bacterial species cross-reacted to varying degrees with one to five H. influenzae antigens.
Original language | English |
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Journal | Acta pathologica et microbiologica Scandinavica. Section B, Microbiology |
Volume | 87 |
Issue number | 6 |
Pages (from-to) | 337-44 |
Number of pages | 8 |
ISSN | 0105-0656 |
DOIs | |
Publication status | Published - Dec 1979 |
Externally published | Yes |
Keywords
- Antigens, Bacterial/analysis
- Bacteria/immunology
- Cross Reactions
- Epitopes
- Haemophilus influenzae/immunology
- Immunoelectrophoresis, Two-Dimensional/methods
- Polysaccharides, Bacterial/immunology
- Species Specificity