Control of fetal growth and neonatal survival by the RasGAP-associated endoribonuclease G3BP

Latifa Zekri; Karim Chebli; Hélène Tourrière; Finn C Nielsen; Thomas V O Hansen; Abdelhaq Rami; Jamal Tazi

doi:10.1128/MCB.25.19.8703-8716.2005

Control of fetal growth and neonatal survival by the RasGAP-associated endoribonuclease G3BP

Latifa Zekri, Karim Chebli, Hélène Tourrière, Finn C Nielsen, Thomas V O Hansen, Abdelhaq Rami, Jamal Tazi

Department of Clinical Biochemistry

81 Citations (Scopus)

Abstract

The regulation of mRNA stability plays a major role in the control of gene expression during cell proliferation, differentiation, and development. Here, we show that inactivation of the RasGAP-associated endoribonuclease (G3BP)-encoding gene leads to embryonic lethality and growth retardation. G3BP-/- mice that survived to term exhibited increased apoptotic cell death in the central nervous system and neonatal lethality. Both in mouse embryonic fibroblasts and during development, the absence of G3BP altered the expression of essential growth factors, among which imprinted gene products and growth arrest-specific mRNAs were outstanding. The results demonstrate that G3BP is essential for proper embryonic growth and development by mediating the coordinate expression of multiple imprinted growth-regulatory transcripts.

Original language	English
Journal	Molecular and Cellular Biology
Volume	25
Issue number	19
Pages (from-to)	8703-16
Number of pages	14
ISSN	0270-7306
DOIs	https://doi.org/10.1128/MCB.25.19.8703-8716.2005
Publication status	Published - Oct 2005

Keywords

Alleles
Animals
Apoptosis
Carrier Proteins/metabolism
Cell Death
Cell Proliferation
Central Nervous System/metabolism
DNA Helicases
Dactinomycin/pharmacology
Female
Fibroblasts/metabolism
Gene Expression Regulation, Developmental
Genetic Vectors
Genotype
Heterozygote
Immunoprecipitation
In Situ Hybridization
Kinetics
Male
Mice
Mice, Knockout
Models, Genetic
Neurons/metabolism
Oligonucleotide Array Sequence Analysis
Poly-ADP-Ribose Binding Proteins
Proteome
RNA Helicases
RNA Recognition Motif Proteins
RNA, Messenger/metabolism
Recombinant Proteins/chemistry
Recombination, Genetic
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Time Factors

Access to Document

10.1128/MCB.25.19.8703-8716.2005

Cite this

@article{63740c23fb824d59970b0939a7cd33e9,

title = "Control of fetal growth and neonatal survival by the RasGAP-associated endoribonuclease G3BP",

abstract = "The regulation of mRNA stability plays a major role in the control of gene expression during cell proliferation, differentiation, and development. Here, we show that inactivation of the RasGAP-associated endoribonuclease (G3BP)-encoding gene leads to embryonic lethality and growth retardation. G3BP-/- mice that survived to term exhibited increased apoptotic cell death in the central nervous system and neonatal lethality. Both in mouse embryonic fibroblasts and during development, the absence of G3BP altered the expression of essential growth factors, among which imprinted gene products and growth arrest-specific mRNAs were outstanding. The results demonstrate that G3BP is essential for proper embryonic growth and development by mediating the coordinate expression of multiple imprinted growth-regulatory transcripts.",

keywords = "Alleles, Animals, Apoptosis, Carrier Proteins/metabolism, Cell Death, Cell Proliferation, Central Nervous System/metabolism, DNA Helicases, Dactinomycin/pharmacology, Female, Fibroblasts/metabolism, Gene Expression Regulation, Developmental, Genetic Vectors, Genotype, Heterozygote, Immunoprecipitation, In Situ Hybridization, Kinetics, Male, Mice, Mice, Knockout, Models, Genetic, Neurons/metabolism, Oligonucleotide Array Sequence Analysis, Poly-ADP-Ribose Binding Proteins, Proteome, RNA Helicases, RNA Recognition Motif Proteins, RNA, Messenger/metabolism, Recombinant Proteins/chemistry, Recombination, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Time Factors",

author = "Latifa Zekri and Karim Chebli and H{\'e}l{\`e}ne Tourri{\`e}re and Nielsen, \{Finn C\} and Hansen, \{Thomas V O\} and Abdelhaq Rami and Jamal Tazi",

year = "2005",

month = oct,

doi = "10.1128/MCB.25.19.8703-8716.2005",

language = "English",

volume = "25",

pages = "8703--16",

journal = "Molecular and Cellular Biology",

issn = "0270-7306",

publisher = "Taylor and Francis Ltd.",

number = "19",

}

TY - JOUR

T1 - Control of fetal growth and neonatal survival by the RasGAP-associated endoribonuclease G3BP

AU - Zekri, Latifa

AU - Chebli, Karim

AU - Tourrière, Hélène

AU - Nielsen, Finn C

AU - Hansen, Thomas V O

AU - Rami, Abdelhaq

AU - Tazi, Jamal

PY - 2005/10

Y1 - 2005/10

N2 - The regulation of mRNA stability plays a major role in the control of gene expression during cell proliferation, differentiation, and development. Here, we show that inactivation of the RasGAP-associated endoribonuclease (G3BP)-encoding gene leads to embryonic lethality and growth retardation. G3BP-/- mice that survived to term exhibited increased apoptotic cell death in the central nervous system and neonatal lethality. Both in mouse embryonic fibroblasts and during development, the absence of G3BP altered the expression of essential growth factors, among which imprinted gene products and growth arrest-specific mRNAs were outstanding. The results demonstrate that G3BP is essential for proper embryonic growth and development by mediating the coordinate expression of multiple imprinted growth-regulatory transcripts.

AB - The regulation of mRNA stability plays a major role in the control of gene expression during cell proliferation, differentiation, and development. Here, we show that inactivation of the RasGAP-associated endoribonuclease (G3BP)-encoding gene leads to embryonic lethality and growth retardation. G3BP-/- mice that survived to term exhibited increased apoptotic cell death in the central nervous system and neonatal lethality. Both in mouse embryonic fibroblasts and during development, the absence of G3BP altered the expression of essential growth factors, among which imprinted gene products and growth arrest-specific mRNAs were outstanding. The results demonstrate that G3BP is essential for proper embryonic growth and development by mediating the coordinate expression of multiple imprinted growth-regulatory transcripts.

KW - Alleles

KW - Animals

KW - Apoptosis

KW - Carrier Proteins/metabolism

KW - Cell Death

KW - Cell Proliferation

KW - Central Nervous System/metabolism

KW - DNA Helicases

KW - Dactinomycin/pharmacology

KW - Female

KW - Fibroblasts/metabolism

KW - Gene Expression Regulation, Developmental

KW - Genetic Vectors

KW - Genotype

KW - Heterozygote

KW - Immunoprecipitation

KW - In Situ Hybridization

KW - Kinetics

KW - Male

KW - Mice

KW - Mice, Knockout

KW - Models, Genetic

KW - Neurons/metabolism

KW - Oligonucleotide Array Sequence Analysis

KW - Poly-ADP-Ribose Binding Proteins

KW - Proteome

KW - RNA Helicases

KW - RNA Recognition Motif Proteins

KW - RNA, Messenger/metabolism

KW - Recombinant Proteins/chemistry

KW - Recombination, Genetic

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Signal Transduction

KW - Time Factors

UR - https://www.scopus.com/pages/publications/25444523445

U2 - 10.1128/MCB.25.19.8703-8716.2005

DO - 10.1128/MCB.25.19.8703-8716.2005

M3 - Journal article

C2 - 16166649

SN - 0270-7306

VL - 25

SP - 8703

EP - 8716

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

IS - 19

ER -

Control of fetal growth and neonatal survival by the RasGAP-associated endoribonuclease G3BP

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this