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Comparison of styrene-divinylbenzene-based monoliths and Vydac nano-liquid chromatography columns for protein analysis

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@article{bcd1e4a8ecff4d4ea2374c223f8c3cbb,
title = "Comparison of styrene-divinylbenzene-based monoliths and Vydac nano-liquid chromatography columns for protein analysis",
abstract = "This report details the fabrication of polystyrene-based nano-LC monolithic columns for the separation of protein molecules. The report focuses on the practical advantages of monolithic columns when compared to conventional packed columns. Capillary columns were made to 100 and 50 microm i.d. and used to analyse a mixture of proteins, these separations were then compared with a conventional protein phase under the same conditions. A second functionalised monolithic polystyrene-based column was also manufactured and compared for the analysis of proteins, under the same analytical conditions, to the standard non-functionalised bare polystyrene monolith. Nano-LC polystyrene monolithic columns were found to be advantageous to conventional phases for the analysis of protein molecules, with a one-step fabrication process, faster analysis times, lower limits of detection hence higher sensitivity.",
keywords = "Chromatography, Liquid/methods, Microscopy, Electron, Scanning, Styrene/chemistry, Vinyl Compounds/chemistry",
author = "Cristina Legido-Quigley and Nicola Marlin and Smith, {Norman William}",
year = "2004",
month = "3",
day = "19",
language = "English",
volume = "1030",
pages = "195--200",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier BV",
number = "1-2",

}

RIS

TY - JOUR

T1 - Comparison of styrene-divinylbenzene-based monoliths and Vydac nano-liquid chromatography columns for protein analysis

AU - Legido-Quigley, Cristina

AU - Marlin, Nicola

AU - Smith, Norman William

PY - 2004/3/19

Y1 - 2004/3/19

N2 - This report details the fabrication of polystyrene-based nano-LC monolithic columns for the separation of protein molecules. The report focuses on the practical advantages of monolithic columns when compared to conventional packed columns. Capillary columns were made to 100 and 50 microm i.d. and used to analyse a mixture of proteins, these separations were then compared with a conventional protein phase under the same conditions. A second functionalised monolithic polystyrene-based column was also manufactured and compared for the analysis of proteins, under the same analytical conditions, to the standard non-functionalised bare polystyrene monolith. Nano-LC polystyrene monolithic columns were found to be advantageous to conventional phases for the analysis of protein molecules, with a one-step fabrication process, faster analysis times, lower limits of detection hence higher sensitivity.

AB - This report details the fabrication of polystyrene-based nano-LC monolithic columns for the separation of protein molecules. The report focuses on the practical advantages of monolithic columns when compared to conventional packed columns. Capillary columns were made to 100 and 50 microm i.d. and used to analyse a mixture of proteins, these separations were then compared with a conventional protein phase under the same conditions. A second functionalised monolithic polystyrene-based column was also manufactured and compared for the analysis of proteins, under the same analytical conditions, to the standard non-functionalised bare polystyrene monolith. Nano-LC polystyrene monolithic columns were found to be advantageous to conventional phases for the analysis of protein molecules, with a one-step fabrication process, faster analysis times, lower limits of detection hence higher sensitivity.

KW - Chromatography, Liquid/methods

KW - Microscopy, Electron, Scanning

KW - Styrene/chemistry

KW - Vinyl Compounds/chemistry

M3 - Journal article

VL - 1030

SP - 195

EP - 200

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

IS - 1-2

ER -

ID: 54963082