Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Comparing Whole-Genome Sequencing with Sanger Sequencing for spa Typing of Methicillin-Resistant Staphylococcus aureus

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Diagnostic Accuracy of Interferon Gamma-Induced Protein 10 mRNA Release Assay for Tuberculosis

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Multicenter evaluation of the QIAstat-Dx respiratory panel for detection of viruses and bacteria in nasopharyngeal swab specimens

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Update from a twelve-year nationwide fungaemia surveillance: increasing intrinsic and acquired resistance causes concern

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. PREVALENCE OF HUMAN PAPILLOMAVIRUS IN SELF-TAKEN SAMPLES FROM SCREENING NON-ATTENDERS

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Dientamoeba fragilis - a Commensal in Children in Danish Day Care Centers

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Antibodies at Delivery in Women, Partners, and Newborns

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Effect of α-Hemolysin Producing E. coli in Two Different Mouse Strains in a DSS Model of Inflammatory Bowel Disease

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. ResFinder 4.0 for predictions of phenotypes from genotypes

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

spa typing of methicillin-resistant Staphylococcus aureus (MRSA) has traditionally been done by PCR amplification and Sanger sequencing of the spa repeat region. At Hvidovre Hospital, Denmark, whole-genome sequencing (WGS) of all MRSA isolates has been performed routinely since January 2013, and an in-house analysis pipeline determines the spa types. Due to national surveillance, all MRSA isolates are sent to Statens Serum Institut, where the spa type is determined by PCR and Sanger sequencing. The purpose of this study was to evaluate the reliability of the spa types obtained by 150-bp paired-end Illumina WGS. MRSA isolates from new MRSA patients in 2013 (n = 699) in the capital region of Denmark were included. We found a 97% agreement between spa types obtained by the two methods. All isolates achieved a spa type by both methods. Nineteen isolates differed in spa types by the two methods, in most cases due to the lack of 24-bp repeats in the whole-genome-sequenced isolates. These related but incorrect spa types should have no consequence in outbreak investigations, since all epidemiologically linked isolates, regardless of spa type, will be included in the single nucleotide polymorphism (SNP) analysis. This will reveal the close relatedness of the spa types. In conclusion, our data show that WGS is a reliable method to determine the spa type of MRSA.

Original languageEnglish
JournalJournal of Clinical Microbiology
Volume52
Issue number12
Pages (from-to)4305-8
Number of pages4
ISSN0095-1137
DOIs
Publication statusPublished - 2014

ID: 44744548