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Comparative Studies of the Gut Microbiota in the Offspring of Mothers With and Without Gestational Diabetes

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@article{e9626afc275c4e1daf7b7a4c3ce0b4b9,
title = "Comparative Studies of the Gut Microbiota in the Offspring of Mothers With and Without Gestational Diabetes",
abstract = "Background: Offspring of mothers with gestational diabetes mellitus (GDM) have increased risk of developing metabolic disorders as they grow up. Microbial colonization of the newborn gut and environmental exposures affecting the configuration of the gut microbiota during infancy have been linked to increased risk of developing disease during childhood and adulthood. In a convenience sample, we examined whether the intestinal tract of children born to mothers with GDM is differentially colonized in early life compared to offspring of mothers with normal gestational glucose regulation. Secondly, we examined whether any such difference persists during infancy, thus potentially conferring increased risk of developing metabolic disease later in life. Methods: Fecal samples were collected from children of mothers with (n = 43) and without GDM (n = 82) during the first week of life and again at an average age of 9 months. The gut microbiota was characterized by 16S rRNA gene amplicon sequencing (V1-V2). Differences in diversity and composition according to maternal GDM status were assessed, addressing potential confounding by mode of delivery, perinatal antibiotics treatment, feeding and infant sex. Results: Children of mothers with GDM were featured by a differential composition of the gut microbiota, both during the first week of life and at 9 months, at higher taxonomic and OTU levels. Sixteen and 15 OTUs were differentially abundant after correction for multiple testing during the first week of life and at 9 months, respectively. Two OTUs remained differentially abundant after adjustment for potential confounders both during the first week of life and at 9 months. Richness (OTU) was decreased in neonates born to mothers with GDM; however, at 9 months no difference in richness was observed. There was no difference in Shannon's diversity or Pielou's evenness at any timepoint. Longitudinally, we detected differential changes in the gut microbiota composition from birth to infancy according to GDM status. Conclusion: Differences in glycaemic regulation in late pregnancy is linked with relatively modest variation in the gut microbiota composition of the offspring during the first week of life and 9 months after birth.",
keywords = "bacterial genera, gestational diabetes, gut microbiota, infancy, maternal glycaemic traits",
author = "Crusell, {Mie Korslund Wiinblad} and Hansen, {Tue Haldor} and Trine Nielsen and Allin, {Kristine H{\o}jgaard} and R{\"u}hlemann, {Malte C} and Peter Damm and Henrik Vestergaard and Christina R{\o}rbye and J{\o}rgensen, {Niklas Rye} and Christiansen, {Ole Bjarne} and Femke-Anouska Heinsen and Andre Franke and Torben Hansen and Jeannet Lauenborg and Oluf Pedersen",
note = "Copyright {\textcopyright} 2020 Crusell, Hansen, Nielsen, Allin, R{\"u}hlemann, Damm, Vestergaard, R{\o}rbye, J{\o}rgensen, Christiansen, Heinsen, Franke, Hansen, Lauenborg and Pedersen.",
year = "2020",
doi = "10.3389/fcimb.2020.536282",
language = "English",
volume = "10",
pages = "536282",
journal = "Frontiers in cellular and infection microbiology",
issn = "2235-2988",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Comparative Studies of the Gut Microbiota in the Offspring of Mothers With and Without Gestational Diabetes

AU - Crusell, Mie Korslund Wiinblad

AU - Hansen, Tue Haldor

AU - Nielsen, Trine

AU - Allin, Kristine Højgaard

AU - Rühlemann, Malte C

AU - Damm, Peter

AU - Vestergaard, Henrik

AU - Rørbye, Christina

AU - Jørgensen, Niklas Rye

AU - Christiansen, Ole Bjarne

AU - Heinsen, Femke-Anouska

AU - Franke, Andre

AU - Hansen, Torben

AU - Lauenborg, Jeannet

AU - Pedersen, Oluf

N1 - Copyright © 2020 Crusell, Hansen, Nielsen, Allin, Rühlemann, Damm, Vestergaard, Rørbye, Jørgensen, Christiansen, Heinsen, Franke, Hansen, Lauenborg and Pedersen.

PY - 2020

Y1 - 2020

N2 - Background: Offspring of mothers with gestational diabetes mellitus (GDM) have increased risk of developing metabolic disorders as they grow up. Microbial colonization of the newborn gut and environmental exposures affecting the configuration of the gut microbiota during infancy have been linked to increased risk of developing disease during childhood and adulthood. In a convenience sample, we examined whether the intestinal tract of children born to mothers with GDM is differentially colonized in early life compared to offspring of mothers with normal gestational glucose regulation. Secondly, we examined whether any such difference persists during infancy, thus potentially conferring increased risk of developing metabolic disease later in life. Methods: Fecal samples were collected from children of mothers with (n = 43) and without GDM (n = 82) during the first week of life and again at an average age of 9 months. The gut microbiota was characterized by 16S rRNA gene amplicon sequencing (V1-V2). Differences in diversity and composition according to maternal GDM status were assessed, addressing potential confounding by mode of delivery, perinatal antibiotics treatment, feeding and infant sex. Results: Children of mothers with GDM were featured by a differential composition of the gut microbiota, both during the first week of life and at 9 months, at higher taxonomic and OTU levels. Sixteen and 15 OTUs were differentially abundant after correction for multiple testing during the first week of life and at 9 months, respectively. Two OTUs remained differentially abundant after adjustment for potential confounders both during the first week of life and at 9 months. Richness (OTU) was decreased in neonates born to mothers with GDM; however, at 9 months no difference in richness was observed. There was no difference in Shannon's diversity or Pielou's evenness at any timepoint. Longitudinally, we detected differential changes in the gut microbiota composition from birth to infancy according to GDM status. Conclusion: Differences in glycaemic regulation in late pregnancy is linked with relatively modest variation in the gut microbiota composition of the offspring during the first week of life and 9 months after birth.

AB - Background: Offspring of mothers with gestational diabetes mellitus (GDM) have increased risk of developing metabolic disorders as they grow up. Microbial colonization of the newborn gut and environmental exposures affecting the configuration of the gut microbiota during infancy have been linked to increased risk of developing disease during childhood and adulthood. In a convenience sample, we examined whether the intestinal tract of children born to mothers with GDM is differentially colonized in early life compared to offspring of mothers with normal gestational glucose regulation. Secondly, we examined whether any such difference persists during infancy, thus potentially conferring increased risk of developing metabolic disease later in life. Methods: Fecal samples were collected from children of mothers with (n = 43) and without GDM (n = 82) during the first week of life and again at an average age of 9 months. The gut microbiota was characterized by 16S rRNA gene amplicon sequencing (V1-V2). Differences in diversity and composition according to maternal GDM status were assessed, addressing potential confounding by mode of delivery, perinatal antibiotics treatment, feeding and infant sex. Results: Children of mothers with GDM were featured by a differential composition of the gut microbiota, both during the first week of life and at 9 months, at higher taxonomic and OTU levels. Sixteen and 15 OTUs were differentially abundant after correction for multiple testing during the first week of life and at 9 months, respectively. Two OTUs remained differentially abundant after adjustment for potential confounders both during the first week of life and at 9 months. Richness (OTU) was decreased in neonates born to mothers with GDM; however, at 9 months no difference in richness was observed. There was no difference in Shannon's diversity or Pielou's evenness at any timepoint. Longitudinally, we detected differential changes in the gut microbiota composition from birth to infancy according to GDM status. Conclusion: Differences in glycaemic regulation in late pregnancy is linked with relatively modest variation in the gut microbiota composition of the offspring during the first week of life and 9 months after birth.

KW - bacterial genera

KW - gestational diabetes

KW - gut microbiota

KW - infancy

KW - maternal glycaemic traits

UR - http://www.scopus.com/inward/record.url?scp=85095581884&partnerID=8YFLogxK

U2 - 10.3389/fcimb.2020.536282

DO - 10.3389/fcimb.2020.536282

M3 - Journal article

C2 - 33194786

VL - 10

SP - 536282

JO - Frontiers in cellular and infection microbiology

JF - Frontiers in cellular and infection microbiology

SN - 2235-2988

M1 - 536282

ER -

ID: 61250832