Co-localisation of the Kir6.2/SUR1 channel complex with glucagon-like peptide-1 and glucose-dependent insulinotrophic polypeptide expression in human ileal cells and implications for glycaemic control in new onset type 1 diabetes

Lotte B Nielsen; Kenneth B Ploug; Peter Swift; Cathrine Ørskov; Inger Jansen-Olesen; Francesco Chiarelli; Jens Juul Holst; Philip Hougaard; Sven Pörksen; Reinhard Holl; Carine de Beaufort; Steen Gammeltoft; Patrik Rorsman; Henrik B Mortensen; Lars Hansen; Hvidøre Study Group

doi:10.1530/EJE-06-0756

Co-localisation of the Kir6.2/SUR1 channel complex with glucagon-like peptide-1 and glucose-dependent insulinotrophic polypeptide expression in human ileal cells and implications for glycaemic control in new onset type 1 diabetes

Lotte B Nielsen, Kenneth B Ploug, Peter Swift, Cathrine Ørskov, Inger Jansen-Olesen, Francesco Chiarelli, Jens Juul Holst, Philip Hougaard, Sven Pörksen, Reinhard Holl, Carine de Beaufort, Steen Gammeltoft, Patrik Rorsman, Henrik B Mortensen, Lars Hansen, Hvidøre Study Group

Department of Paediatrics

60 Citations (Scopus)

Abstract

The ATP-dependent K+-channel (K(ATP)) is critical for glucose sensing and normal glucagon and insulin secretion from pancreatic endocrine alpha- and beta-cells. Gastrointestinal endocrine L- and K-cells are also glucose-sensing cells secreting glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotrophic polypeptide (GIP) respectively. The aims of this study were to 1) investigate the expression and co-localisation of the K(ATP) channel subunits, Kir6.2 and SUR1, in human L- and K-cells and 2) investigate if a common hyperactive variant of the Kir6.2 subunit, Glu23Lys, exerts a functional impact on glucose-sensing tissues in vivo that may affect the overall glycaemic control in children with new-onset type 1 diabetes.

Original language	English
Journal	European Journal of Endocrinology
Volume	156
Issue number	6
Pages (from-to)	663-71
Number of pages	9
ISSN	0804-4643
DOIs	https://doi.org/10.1530/EJE-06-0756
Publication status	Published - 2007

Keywords

ATP-Binding Cassette Transporters
Adolescent
Blotting, Western
C-Peptide
Child
Diabetes Mellitus, Type 1
Eating
Female
Gastric Inhibitory Polypeptide
Genotype
Glucagon
Glucagon-Like Peptide 1
Hemoglobin A, Glycosylated
Humans
Hyperglycemia
Hypoglycemic Agents
Ileum
Immunohistochemistry
Insulin
Islets of Langerhans
Male
Polymorphism, Restriction Fragment Length
Potassium Channels
Potassium Channels, Inwardly Rectifying
Receptors, Drug

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Nielsen, L. B., Ploug, K. B., Swift, P., Ørskov, C., Jansen-Olesen, I., Chiarelli, F., Holst, J. J., Hougaard, P., Pörksen, S., Holl, R., de Beaufort, C., Gammeltoft, S., Rorsman, P., Mortensen, H. B., Hansen, L., & Hvidøre Study Group (2007). Co-localisation of the Kir6.2/SUR1 channel complex with glucagon-like peptide-1 and glucose-dependent insulinotrophic polypeptide expression in human ileal cells and implications for glycaemic control in new onset type 1 diabetes. European Journal of Endocrinology, 156(6), 663-71. https://doi.org/10.1530/EJE-06-0756

Co-localisation of the Kir6.2/SUR1 channel complex with glucagon-like peptide-1 and glucose-dependent insulinotrophic polypeptide expression in human ileal cells and implications for glycaemic control in new onset type 1 diabetes. / Nielsen, Lotte B; Ploug, Kenneth B; Swift, Peter et al.
In: European Journal of Endocrinology, Vol. 156, No. 6, 2007, p. 663-71.

Research output: Contribution to journal › Journal article › Research › peer-review

Nielsen, LB, Ploug, KB, Swift, P, Ørskov, C, Jansen-Olesen, I, Chiarelli, F, Holst, JJ, Hougaard, P, Pörksen, S, Holl, R, de Beaufort, C, Gammeltoft, S, Rorsman, P, Mortensen, HB, Hansen, L & Hvidøre Study Group 2007, 'Co-localisation of the Kir6.2/SUR1 channel complex with glucagon-like peptide-1 and glucose-dependent insulinotrophic polypeptide expression in human ileal cells and implications for glycaemic control in new onset type 1 diabetes', European Journal of Endocrinology, vol. 156, no. 6, pp. 663-71. https://doi.org/10.1530/EJE-06-0756

Nielsen LB, Ploug KB, Swift P, Ørskov C, Jansen-Olesen I, Chiarelli F et al. Co-localisation of the Kir6.2/SUR1 channel complex with glucagon-like peptide-1 and glucose-dependent insulinotrophic polypeptide expression in human ileal cells and implications for glycaemic control in new onset type 1 diabetes. European Journal of Endocrinology. 2007;156(6):663-71. doi: 10.1530/EJE-06-0756

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title = "Co-localisation of the Kir6.2/SUR1 channel complex with glucagon-like peptide-1 and glucose-dependent insulinotrophic polypeptide expression in human ileal cells and implications for glycaemic control in new onset type 1 diabetes",

abstract = "The ATP-dependent K+-channel (K(ATP)) is critical for glucose sensing and normal glucagon and insulin secretion from pancreatic endocrine alpha- and beta-cells. Gastrointestinal endocrine L- and K-cells are also glucose-sensing cells secreting glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotrophic polypeptide (GIP) respectively. The aims of this study were to 1) investigate the expression and co-localisation of the K(ATP) channel subunits, Kir6.2 and SUR1, in human L- and K-cells and 2) investigate if a common hyperactive variant of the Kir6.2 subunit, Glu23Lys, exerts a functional impact on glucose-sensing tissues in vivo that may affect the overall glycaemic control in children with new-onset type 1 diabetes.",

keywords = "ATP-Binding Cassette Transporters, Adolescent, Blotting, Western, C-Peptide, Child, Diabetes Mellitus, Type 1, Eating, Female, Gastric Inhibitory Polypeptide, Genotype, Glucagon, Glucagon-Like Peptide 1, Hemoglobin A, Glycosylated, Humans, Hyperglycemia, Hypoglycemic Agents, Ileum, Immunohistochemistry, Insulin, Islets of Langerhans, Male, Polymorphism, Restriction Fragment Length, Potassium Channels, Potassium Channels, Inwardly Rectifying, Receptors, Drug",

author = "Nielsen, \{Lotte B\} and Ploug, \{Kenneth B\} and Peter Swift and Cathrine {\O}rskov and Inger Jansen-Olesen and Francesco Chiarelli and Holst, \{Jens Juul\} and Philip Hougaard and Sven P{\"o}rksen and Reinhard Holl and \{de Beaufort\}, Carine and Steen Gammeltoft and Patrik Rorsman and Mortensen, \{Henrik B\} and Lars Hansen and \{Hvid{\o}re Study Group\}",

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doi = "10.1530/EJE-06-0756",

language = "English",

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T1 - Co-localisation of the Kir6.2/SUR1 channel complex with glucagon-like peptide-1 and glucose-dependent insulinotrophic polypeptide expression in human ileal cells and implications for glycaemic control in new onset type 1 diabetes

AU - Nielsen, Lotte B

AU - Ploug, Kenneth B

AU - Swift, Peter

AU - Ørskov, Cathrine

AU - Jansen-Olesen, Inger

AU - Chiarelli, Francesco

AU - Holst, Jens Juul

AU - Hougaard, Philip

AU - Pörksen, Sven

AU - Holl, Reinhard

AU - de Beaufort, Carine

AU - Gammeltoft, Steen

AU - Rorsman, Patrik

AU - Mortensen, Henrik B

AU - Hansen, Lars

AU - Hvidøre Study Group

PY - 2007

Y1 - 2007

N2 - The ATP-dependent K+-channel (K(ATP)) is critical for glucose sensing and normal glucagon and insulin secretion from pancreatic endocrine alpha- and beta-cells. Gastrointestinal endocrine L- and K-cells are also glucose-sensing cells secreting glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotrophic polypeptide (GIP) respectively. The aims of this study were to 1) investigate the expression and co-localisation of the K(ATP) channel subunits, Kir6.2 and SUR1, in human L- and K-cells and 2) investigate if a common hyperactive variant of the Kir6.2 subunit, Glu23Lys, exerts a functional impact on glucose-sensing tissues in vivo that may affect the overall glycaemic control in children with new-onset type 1 diabetes.

AB - The ATP-dependent K+-channel (K(ATP)) is critical for glucose sensing and normal glucagon and insulin secretion from pancreatic endocrine alpha- and beta-cells. Gastrointestinal endocrine L- and K-cells are also glucose-sensing cells secreting glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotrophic polypeptide (GIP) respectively. The aims of this study were to 1) investigate the expression and co-localisation of the K(ATP) channel subunits, Kir6.2 and SUR1, in human L- and K-cells and 2) investigate if a common hyperactive variant of the Kir6.2 subunit, Glu23Lys, exerts a functional impact on glucose-sensing tissues in vivo that may affect the overall glycaemic control in children with new-onset type 1 diabetes.

KW - ATP-Binding Cassette Transporters

KW - Adolescent

KW - Blotting, Western

KW - C-Peptide

KW - Child

KW - Diabetes Mellitus, Type 1

KW - Eating

KW - Female

KW - Gastric Inhibitory Polypeptide

KW - Genotype

KW - Glucagon

KW - Glucagon-Like Peptide 1

KW - Hemoglobin A, Glycosylated

KW - Humans

KW - Hyperglycemia

KW - Hypoglycemic Agents

KW - Ileum

KW - Immunohistochemistry

KW - Insulin

KW - Islets of Langerhans

KW - Male

KW - Polymorphism, Restriction Fragment Length

KW - Potassium Channels

KW - Potassium Channels, Inwardly Rectifying

KW - Receptors, Drug

UR - https://www.scopus.com/pages/publications/34250756516

U2 - 10.1530/EJE-06-0756

DO - 10.1530/EJE-06-0756

M3 - Journal article

C2 - 17535866

SN - 0804-4643

VL - 156

SP - 663

EP - 671

JO - European Journal of Endocrinology

JF - European Journal of Endocrinology

IS - 6

ER -

Co-localisation of the Kir6.2/SUR1 channel complex with glucagon-like peptide-1 and glucose-dependent insulinotrophic polypeptide expression in human ileal cells and implications for glycaemic control in new onset type 1 diabetes

Abstract

Keywords

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