Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

Munsoor A Hanifa; Raluca G Maltesen; Bodil S Rasmussen; Katrine B Buggeskov; Hanne B Ravn; Martin Skott; Søren Nielsen; Jørgen Frøkiær; Troels Ring; Reinhard Wimmer

doi:10.1007/s11306-019-1629-8

Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

Munsoor A Hanifa, Raluca G Maltesen, Bodil S Rasmussen, Katrine B Buggeskov, Hanne B Ravn, Martin Skott, Søren Nielsen, Jørgen Frøkiær, Troels Ring, Reinhard Wimmer

Department of Thoracic Anaesthesiology

Abstract

BACKGROUND: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities.

AIMS: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies.

METHODS: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated.

RESULTS: Integral changes were generally small, but serum citrate signals decreased significantly in some samples.

CONCLUSIONS: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.

Original language	English
Journal	Metabolomics
Volume	16
Issue number	1
Pages (from-to)	7
ISSN	1573-3882
DOIs	https://doi.org/10.1007/s11306-019-1629-8
Publication status	Published - 2019

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title = "Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra",

abstract = "BACKGROUND: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities.AIMS: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies.METHODS: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated.RESULTS: Integral changes were generally small, but serum citrate signals decreased significantly in some samples.CONCLUSIONS: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.",

author = "Hanifa, {Munsoor A} and Maltesen, {Raluca G} and Rasmussen, {Bodil S} and Buggeskov, {Katrine B} and Ravn, {Hanne B} and Martin Skott and S{\o}ren Nielsen and J{\o}rgen Fr{\o}ki{\ae}r and Troels Ring and Reinhard Wimmer",

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doi = "10.1007/s11306-019-1629-8",

language = "English",

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journal = "Metabolomics",

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TY - JOUR

T1 - Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

AU - Hanifa, Munsoor A

AU - Maltesen, Raluca G

AU - Rasmussen, Bodil S

AU - Buggeskov, Katrine B

AU - Ravn, Hanne B

AU - Skott, Martin

AU - Nielsen, Søren

AU - Frøkiær, Jørgen

AU - Ring, Troels

AU - Wimmer, Reinhard

PY - 2019

Y1 - 2019

N2 - BACKGROUND: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities.AIMS: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies.METHODS: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated.RESULTS: Integral changes were generally small, but serum citrate signals decreased significantly in some samples.CONCLUSIONS: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.

AB - BACKGROUND: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities.AIMS: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies.METHODS: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated.RESULTS: Integral changes were generally small, but serum citrate signals decreased significantly in some samples.CONCLUSIONS: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.

U2 - 10.1007/s11306-019-1629-8

DO - 10.1007/s11306-019-1629-8

M3 - Journal article

C2 - 31858270

VL - 16

SP - 7

JO - Metabolomics

JF - Metabolomics

SN - 1573-3882

IS - 1

ER -

Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

Abstract

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