Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Circulating cell-free nucleosomes as biomarkers for early detection of colorectal cancer

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Real-world evidence to guide healthcare policies in oncology

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Phospho-ERK levels as predictors for chemotherapy of rectal carcinoma

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Circulating tumor DNA as a marker of treatment response in BRAF V600E mutated non-melanoma solid tumors

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Genome-wide cell-free DNA fragmentation in patients with cancer

    Research output: Contribution to journalLetterResearchpeer-review

  2. Mandibular advancement device therapy for obstructive sleep apnea: a prospective study on predictors of treatment success

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Early metastatic colorectal cancers show increased tissue expression of miR-17/92 cluster members in the invasive tumor front

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Triage for selection to colonoscopy?

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

The aim was to evaluate serum levels of circulating cell-free nucleosomes (ccfn) containing a variety of epigenetic signals including 5-methylcytosine DNA, histone modifications H3K9Me3, H3K9Ac, H3S10PO4, H3K36Me3, H4K20Me3, H4PanAc and pH2AX, nucleosome variant H2AZ and nucleosome adducts with HMGB1 and EZH2 as well as ccfn per se, in addition to develop and evaluate predictor models based on the above mentioned ccfn and including serum levels of carcinoembryonic antigen (CEA), in early detection of colorectal cancer (CRC). Blood-samples were collected from 4,105 individuals undergoing colonoscopy. Serum levels of ccfn and CEA were determined using enzyme-linked immunosorbent assays platforms. Individual assessment of levels of ccfn showed area under the receiver operating characteristic curve (AUCROC) = 0.525-0.576 in discrimination of individuals with CRC from individuals with non-malignant findings. Predictor models including ccfn containing 5-methylcytosine DNA, CEA, age and gender improved results (AUCROC = 0.736, sensitivity = 0.37 at specificity = 0.90). Further improvement was achieved in discrimination of individuals with CRC from individuals with clean colorectum (AUCROC = 0.840, sensitivity = 0.57 at specificity = 0.90). The levels of ccfn among patients with CRC appeared to be stage-independent. In conclusion, the performance of the developed predictor models is potentially promising in early detection of CRC.

Original languageEnglish
JournalOncotarget
Volume9
Issue number12
Pages (from-to)10247-10258
Number of pages12
ISSN1949-2553
DOIs
Publication statusPublished - 17 Oct 2017

ID: 52633189