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Chromosomal beta-lactamase is packaged into membrane vesicles and secreted from Pseudomonas aeruginosa

O Ciofu, T J Beveridge, J Kadurugamuwa, J Walther-Rasmussen, N Høiby

259 Citations (Scopus)

Abstract

Membrane vesicles were isolated from one beta-lactam-sensitive and three beta-lactam-resistant Pseudomonas aeruginosa clinical isolates from patients with cystic fibrosis. The presence of the chromosomally encoded beta-lactamase in the membrane vesicles was shown by electron microscopy and enzymatic studies. This is the first report of extracellular secretion of beta-lactamase in P. aeruginosa and it seems that the enzyme is packaged into membrane vesicles.

Original languageEnglish
JournalThe Journal of antimicrobial chemotherapy
Volume45
Issue number1
Pages (from-to)9-13
Number of pages5
ISSN0305-7453
DOIs
Publication statusPublished - Jan 2000
Externally publishedYes

Keywords

  • Cell Membrane/enzymology
  • Chromosomes, Bacterial/genetics
  • Cystic Fibrosis/microbiology
  • Humans
  • Lung/microbiology
  • Pseudomonas aeruginosa/enzymology
  • beta-Lactam Resistance
  • beta-Lactamases/genetics

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