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Chemokine expression in murine RPE/choroid in response to systemic viral infection and elevated levels of circulating interferon-γ

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@article{6d73a749624246948200145d32e3550c,
title = "Chemokine expression in murine RPE/choroid in response to systemic viral infection and elevated levels of circulating interferon-γ",
abstract = "P URPOSE . To examine how circulating immune mediators in vivo may affect gene and protein expression at the RPE/choroid interface. M ETHODS . Young mice were systemically infected with lymphocytic choriomeningitis virus (LCMV) or continuously infused with IFN-γ. RPE/choroid was isolated and analyzed with whole-transcriptome gene expression microarrays. Selected gene expression findings were validated at the protein level. R ESULTS . Both the systemic immune activation from virus infection and the sterile systemically increased level of IFN-γ resulted in increased expression of chemokine ligands, chemokine receptors, and early complement components in isolates of RPE/choroid. These findings were largely absent from LCMV-infected mice deficient in either the interferon α/β receptor or IFN-γ. C ONCLUSIONS . Together, these findings demonstrate that acute systemic immune activation results in a local response at the RPE/choroid interface that may include chemokine-dependent recruitment of inflammatory cells and engagement of the complement system. This may represent a link between the systemic low-grade inflammation and the retinal pathology observed in several multifactorial entities such as aging, AMD, and diabetes.",
keywords = "Chemokine, Complement, Interferon, LCMV, Retina, RPE",
author = "Carsten Faber and Juel, {Helene B{\ae}k} and Jensen, {Benjamin Anderschou Holbech} and Christensen, {Jan Pravsgaard} and Prause, {Jan Ulrik} and Thomsen, {Allan Randrup} and Nissen, {Mogens Holst}",
year = "2019",
month = "1",
day = "1",
doi = "10.1167/iovs.18-25721",
language = "English",
volume = "60",
pages = "192--201",
journal = "Investigative ophthalmology & visual science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology",
number = "1",

}

RIS

TY - JOUR

T1 - Chemokine expression in murine RPE/choroid in response to systemic viral infection and elevated levels of circulating interferon-γ

AU - Faber, Carsten

AU - Juel, Helene Bæk

AU - Jensen, Benjamin Anderschou Holbech

AU - Christensen, Jan Pravsgaard

AU - Prause, Jan Ulrik

AU - Thomsen, Allan Randrup

AU - Nissen, Mogens Holst

PY - 2019/1/1

Y1 - 2019/1/1

N2 - P URPOSE . To examine how circulating immune mediators in vivo may affect gene and protein expression at the RPE/choroid interface. M ETHODS . Young mice were systemically infected with lymphocytic choriomeningitis virus (LCMV) or continuously infused with IFN-γ. RPE/choroid was isolated and analyzed with whole-transcriptome gene expression microarrays. Selected gene expression findings were validated at the protein level. R ESULTS . Both the systemic immune activation from virus infection and the sterile systemically increased level of IFN-γ resulted in increased expression of chemokine ligands, chemokine receptors, and early complement components in isolates of RPE/choroid. These findings were largely absent from LCMV-infected mice deficient in either the interferon α/β receptor or IFN-γ. C ONCLUSIONS . Together, these findings demonstrate that acute systemic immune activation results in a local response at the RPE/choroid interface that may include chemokine-dependent recruitment of inflammatory cells and engagement of the complement system. This may represent a link between the systemic low-grade inflammation and the retinal pathology observed in several multifactorial entities such as aging, AMD, and diabetes.

AB - P URPOSE . To examine how circulating immune mediators in vivo may affect gene and protein expression at the RPE/choroid interface. M ETHODS . Young mice were systemically infected with lymphocytic choriomeningitis virus (LCMV) or continuously infused with IFN-γ. RPE/choroid was isolated and analyzed with whole-transcriptome gene expression microarrays. Selected gene expression findings were validated at the protein level. R ESULTS . Both the systemic immune activation from virus infection and the sterile systemically increased level of IFN-γ resulted in increased expression of chemokine ligands, chemokine receptors, and early complement components in isolates of RPE/choroid. These findings were largely absent from LCMV-infected mice deficient in either the interferon α/β receptor or IFN-γ. C ONCLUSIONS . Together, these findings demonstrate that acute systemic immune activation results in a local response at the RPE/choroid interface that may include chemokine-dependent recruitment of inflammatory cells and engagement of the complement system. This may represent a link between the systemic low-grade inflammation and the retinal pathology observed in several multifactorial entities such as aging, AMD, and diabetes.

KW - Chemokine

KW - Complement

KW - Interferon

KW - LCMV

KW - Retina

KW - RPE

UR - http://www.scopus.com/inward/record.url?scp=85060130172&partnerID=8YFLogxK

U2 - 10.1167/iovs.18-25721

DO - 10.1167/iovs.18-25721

M3 - Journal article

VL - 60

SP - 192

EP - 201

JO - Investigative ophthalmology & visual science

JF - Investigative ophthalmology & visual science

SN - 0146-0404

IS - 1

ER -

ID: 59475635